Weon Hang-Yeon, Kim Byung-Yong, Kwon Soon-Wo, Go Seung-Joo, Koo Bon-Sung, Stackebrandt Erko
Applied Microbiology Division, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2301-4. doi: 10.1099/ijs.0.65530-0.
A light-yellow-coloured bacterium, designated strain 4T-6(T), was isolated from cotton waste compost in Korea. Cells of the strain were strictly aerobic, Gram-negative, motile (by means of single polar flagella) and rod-shaped. Optimal growth occurred at 30 degrees C and at pH 7.0-8.0. The major fatty acids were 11-methyl C(18 : 1)omega7c (23.8 %), C(18 : 1)omega7c (23.2 %), C(16 : 0) (19.8 %) and C(17 : 0) (14.8 %). The DNA G+C content was 67.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 4T-6(T) was a member of the genus Phenylobacterium, showing the highest sequence similarities with those of 'Phenylobacterium zucineum' HLK1 (98.8 %) and Phenylobacterium lituiforme DSM 14363(T) (98.4 %). However, the values for DNA-DNA relatedness between strain 4T-6(T) and 'P. zucineum' HLK1 and P. lituiforme DSM 14363(T) were 45 and 43 %, respectively. Phylogenetic characteristics, physiological properties and DNA-DNA hybridization data indicate that strain 4T-6(T) represents a novel species of the genus Phenylobacterium, for which the name Phenylobacterium composti sp. nov. is proposed. The type strain is 4T-6(T) (=KACC 12597(T)=DSM 19425(T)).
从韩国的棉花废料堆肥中分离出一株淡黄色细菌，命名为菌株4T-6(T)。该菌株细胞严格需氧，革兰氏阴性，具运动性（借助单根极生鞭毛），呈杆状。最适生长温度为30℃，最适pH值为7.0 - 8.0。主要脂肪酸为11-甲基C(18 : 1)ω7c（23.8%）、C(18 : 1)ω7c（23.2%）、C(16 : 0)（19.8%）和C(17 : 0)（14.8%）。DNA的G+C含量为67.5 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株4T-6(T)是苯基杆菌属的成员，与“西葫芦苯基杆菌”HLK1（98.8%）和弯曲苯基杆菌DSM 14363(T)（98.4%）的序列相似性最高。然而，菌株4T-6(T)与“西葫芦苯基杆菌”HLK1以及弯曲苯基杆菌DSM 14363(T)之间的DNA-DNA相关性值分别为45%和43%。系统发育特征、生理特性和DNA-DNA杂交数据表明，菌株4T-6(T)代表了苯基杆菌属的一个新物种，为此提出新名称为堆肥苯基杆菌（Phenylobacterium composti sp. nov.）。模式菌株为4T-6(T)（=KACC 12597(T)=DSM 19425(T)）。
