Chen Yi-Guang, Cui Xiao-Long, Zhang Yu-Qin, Li Wen-Jun, Wang Yong-Xia, Xu Li-Hua, Peng Qian, Wen Meng-Liang, Jiang Cheng-Lin
Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2008 Oct;58(Pt 10):2403-8. doi: 10.1099/ijs.0.65698-0.
A Gram-positive, moderately halophilic, moderately thermophilic, endospore-forming, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-C55.5(T), was isolated from saline soil in the Qaidam Basin, north-west China. Cells were slender rods, motile by means of peritrichous flagella. Filamentous forms were present throughout the growth cycle. Strain YIM-C55.5(T) was able to grow in the presence of 7-30 % (w/v) NaCl and at pH 6.0-9.0, with optimum growth at 15 % (w/v) NaCl and pH 7.0. It was able to grow at 28-60 degrees C, with optimum growth at 45-50 degrees C. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C(15 : 0), C(16 : 0), anteiso-C(15 : 0), anteiso-C(17 : 0) and C(18 : 0). Strain YIM-C55.5(T) possessed menaquinone 7 (MK-7) as the predominant respiratory quinone and diphosphatidylglycerol and phosphatidylglycerol as polar lipids. The genomic DNA G+C content was 42.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C55.5(T) belonged to the family Bacillaceae and was related most closely to members of the genus Gracilibacillus, but with low levels of similarity to the type strains of recognized species of the genus (Gracilibacillus orientalis XH-63(T), 95.1 %; Gracilibacillus boraciitolerans T-16X(T), 94.0 %; Gracilibacillus dipsosauri DD1(T), 93.7 %; Gracilibacillus halotolerans NN(T), 93.5 %). Together with phenotypic data, these results suggest that strain YIM-C55.5(T) represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus halophilus sp. nov. is proposed. The type strain is YIM-C55.5(T) (=DSM 17856(T) =CGMCC 1.6303(T)).
从中国西北部柴达木盆地的盐土中分离出一株革兰氏阳性、中度嗜盐、中度嗜热、产芽孢、过氧化氢酶和氧化酶阳性、专性需氧的细菌，菌株YIM-C55.5(T)。细胞为细长杆状，借助周生鞭毛运动。在整个生长周期中均存在丝状形态。菌株YIM-C55.5(T)能够在7%-30%（w/v）NaCl和pH 6.0-9.0条件下生长，最适生长条件为15%（w/v）NaCl和pH 7.0。它能够在28-60℃下生长，最适生长温度为45-50℃。细胞壁肽聚糖中存在内消旋二氨基庚二酸。主要细胞脂肪酸为异-C(15 : 0)、C(16 : 0)、anteiso-C(15 : 0)、anteiso-C(17 : 0)和C(18 : 0)。菌株YIM-C55.5(T)以甲基萘醌7（MK-7）作为主要呼吸醌，以二磷脂酰甘油和磷脂酰甘油作为极性脂质。基因组DNA G+C含量为42.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株YIM-C55.5(T)属于芽孢杆菌科，与纤细芽孢杆菌属的成员关系最为密切，但与该属已确认物种的模式菌株相似度较低（东方纤细芽孢杆菌XH-63(T)，95.1%；耐硼纤细芽孢杆菌T-16X(T)，94.0%；双嵴龙纤细芽孢杆菌DD1(T)，93.7%；耐盐纤细芽孢杆菌NN(T)，93.5%）。结合表型数据，这些结果表明菌株YIM-C55.5(T)代表纤细芽孢杆菌属的一个新物种，为此提出新名称嗜盐纤细芽孢杆菌（Gracilibacillus halophilus sp. nov.）。模式菌株为YIM-C55.5(T)（=DSM 17856(T)=CGMCC 1.6303(T)）。
