Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, PR China.
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China.
Int J Syst Evol Microbiol. 2020 Jun;70(6):3701-3710. doi: 10.1099/ijsem.0.004224. Epub 2020 May 15.
A moderately halophilic strain, designated SCU50, was recovered from a saline soil sample and characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain SCU50 belonged to the genus and was most closely related to TP2-8 (98.1 % similarity) and XH-63 (97.7 %). Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized species. The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%. The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C. It contained anteiso-C, iso-C and anteiso-C as the dominant fatty acids and menaquinone-7 as the major respiratory quinone. The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. The cell-wall peptidoglycan type was A1γ based on -diaminopimelic acid. Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50 should be assigned as representing a novel species within the genus . Thus, a novel taxon named sp. nov. was first established, with SCU50 (=CGMCC 1.17336=KCTC 43107) as the type strain.
一株中度嗜盐菌株,命名为 SCU50,从盐土样本中分离得到,并通过多相分类法进行了表征。16S rRNA 基因序列分析表明,菌株 SCU50 属于 属,与 TP2-8(98.1%相似度)和 XH-63(97.7%相似度)最为密切相关。基因组平均核苷酸同一性和数字 DNA-DNA 杂交分析证实,与其他公认的 种相比,新分离株具有独立的种的地位。基因组大小约为 5.09 Mbp,DNA G+C 含量为 36.7 mol%。该菌株在 10-15%(w/v)NaCl、pH 6.5-7.5 和 25-30℃下生长最佳。它含有 anteiso-C、iso-C 和 anteiso-C 作为主要脂肪酸,menaquinone-7 作为主要呼吸醌。检测到极性脂谱,发现包含双磷脂酰甘油、磷脂酰甘油、一种未鉴定的磷脂和一种未鉴定的脂。细胞壁肽聚糖类型为基于 -二氨基庚二酸的 A1γ。综合表型、化学分类、基因组和系统发育特征数据,得出结论,菌株 SCU50 应被归为 属内的一个新种。因此,首次建立了一个新的分类单元,命名为 sp. nov.,SCU50(=CGMCC 1.17336=KCTC 43107)作为模式菌株。