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sp. nov.,一种从中西部盐碱土中分离出的中等嗜盐菌。

sp. nov., a moderate halophile isolated from saline soil in Northwest China.

机构信息

Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, PR China.

College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Jun;70(6):3701-3710. doi: 10.1099/ijsem.0.004224. Epub 2020 May 15.

DOI:10.1099/ijsem.0.004224
PMID:32416748
Abstract

A moderately halophilic strain, designated SCU50, was recovered from a saline soil sample and characterized by a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain SCU50 belonged to the genus and was most closely related to TP2-8 (98.1 % similarity) and XH-63 (97.7 %). Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized species. The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%. The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C. It contained anteiso-C, iso-C and anteiso-C as the dominant fatty acids and menaquinone-7 as the major respiratory quinone. The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. The cell-wall peptidoglycan type was A1γ based on -diaminopimelic acid. Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50 should be assigned as representing a novel species within the genus . Thus, a novel taxon named sp. nov. was first established, with SCU50 (=CGMCC 1.17336=KCTC 43107) as the type strain.

摘要

一株中度嗜盐菌株,命名为 SCU50,从盐土样本中分离得到,并通过多相分类法进行了表征。16S rRNA 基因序列分析表明,菌株 SCU50 属于 属,与 TP2-8(98.1%相似度)和 XH-63(97.7%相似度)最为密切相关。基因组平均核苷酸同一性和数字 DNA-DNA 杂交分析证实,与其他公认的 种相比,新分离株具有独立的种的地位。基因组大小约为 5.09 Mbp,DNA G+C 含量为 36.7 mol%。该菌株在 10-15%(w/v)NaCl、pH 6.5-7.5 和 25-30℃下生长最佳。它含有 anteiso-C、iso-C 和 anteiso-C 作为主要脂肪酸,menaquinone-7 作为主要呼吸醌。检测到极性脂谱,发现包含双磷脂酰甘油、磷脂酰甘油、一种未鉴定的磷脂和一种未鉴定的脂。细胞壁肽聚糖类型为基于 -二氨基庚二酸的 A1γ。综合表型、化学分类、基因组和系统发育特征数据,得出结论,菌株 SCU50 应被归为 属内的一个新种。因此,首次建立了一个新的分类单元,命名为 sp. nov.,SCU50(=CGMCC 1.17336=KCTC 43107)作为模式菌株。

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