Montanari M, Pitzolu G, Felline C, Piana G
Department of Oral Sciences, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
Eur J Paediatr Dent. 2008 Sep;9(3):125-31.
ATM: Oral health is important for everyone, but specially for children and people requiring special care owing to impaired manual ability. Primary prevention, with correct oral hygiene, proper diet, use of fluoride and pit and fissure sealants, can reduce caries risk thereby improving patients' quality of life. The first aim of this paper was to assess the marginal microleakage of different pit and fissure sealants after immersion in a cariogenic solution. The second aim was to evaluate the ability of the materials to penetrate into the bottom of the fissure.
32 posterior teeth were divided into four groups based on the type of sealant tested: Concise, Clinpro, Fissurit, Fissurit F. Samples were immersed and stored in a lactic acid solution (pH 4.4, 0.1 M) at 37 degrees C for different periods: 1 day; 3 days; 7 days; 10 days. Then each sample was stored in erythrosine solution for 24 hours at 37 degrees C. Each sample was sectioned in a mesio-distal direction and evaluated at the stereomicroscope and subsequently at SEM.
SEM analysis showed that Clinpro and Concise obtained a good penetration inside the fissure and a good adaptation to the enamel wall, while Fissurit and Fissurit F showed gaps at the sealant-enamel interface and voids.
Sealant application is an important means for caries prevention but, for a lasting effect, it must be associated with good oral hygiene and regular dental check-ups.
自噬相关蛋白激酶（ATM）：口腔健康对每个人都很重要，对于儿童以及因手部功能受损而需要特殊护理的人群尤为如此。通过正确的口腔卫生、合理饮食、使用氟化物和窝沟封闭剂进行一级预防，可以降低龋齿风险，从而提高患者的生活质量。本文的首要目的是评估不同窝沟封闭剂浸泡在致龋溶液后边缘微渗漏情况。第二个目的是评估材料渗入窝沟底部的能力。
根据所测试的封闭剂类型，将32颗后牙分为四组：Concise、Clinpro、Fissurit、Fissurit F。样本在37摄氏度下浸泡并储存在乳酸溶液（pH 4.4, 0.1 M）中不同时间段：1天；3天；7天；10天。然后每个样本在37摄氏度下于赤藓红溶液中储存24小时。每个样本沿近远中方向切片，先在体视显微镜下评估，随后在扫描电子显微镜下评估。
扫描电子显微镜分析显示，Clinpro和Concise在窝沟内有良好的渗入且与釉质壁贴合良好，而Fissurit和Fissurit F在封闭剂 - 釉质界面处有间隙和空隙。
应用封闭剂是预防龋齿的重要手段，但为了获得持久效果，必须结合良好的口腔卫生和定期的牙科检查。
