Mehrabkhani Maryam, Mazhari Fatemeh, Sadeghi Samaneh, Ebrahimi Masoumeh
Department of Pediatric Dentistry, Dental Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pediatric Dentistry, Dental Material Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Eur J Dent. 2015 Oct-Dec;9(4):558-563. doi: 10.4103/1305-7456.172631.
The aim of this study was to evaluate the effects of enamel or dentin bonding agent (DBA) and sealant viscosity on sealant microleakage.
Sixty extracted human premolars were randomly divided into two equal groups (based on sealant viscosity) and each group was divided into three subgroups of 10 teeth. Group 1 (low viscosity sealant, Seal-Rite, Pulpdent, USA with 7.7% filler): Prophylaxis, enameloplasty, etching of occlusal surfaces with 38% of phosphoric acid gel, rinsing and drying, followed by (1) enamel bonding agent (EBA) (Margin Bond, Coltène/Whaledent AG) or (2) DBA (Excite, Ivoclar Vivadent AG, Liechtenstein) or (3) no bonding (NB) prior to sealant application. In Group 2, similar procedures were performed except for applying a high viscosity sealant (Seal-Rite, Pulpdent, The USA with 34.4% filler). Specimens were thermocycled and then immersed in a 0.5% basic fuchsine solution for 24 h next, buccolingual slices of samples were scored under a stereomicroscope. The Kruskal-Wallis and Mann-Whitney U-tests were used for data analysis.
There was no significant difference between DBA, EBA, and NB subgroups in the microleakage scores in both groups. Low viscosity sealant had a lower microleakage than the high viscosity sealant in both DBA (P = 0.002) and NB (P = 0.041) subgroups.
The results indicated that the use of low viscosity sealant reduced the microleakage of pit and fissure sealants. However, the use of a bonding agent before sealant placement didn't affect the microleakage.
本研究旨在评估釉质或牙本质粘结剂（DBA）以及封闭剂粘度对封闭剂微渗漏的影响。
60颗拔除的人类前磨牙随机分为两组（基于封闭剂粘度），每组再分为3个亚组，每组10颗牙。第1组（低粘度封闭剂，Seal - Rite，美国Pulpdent公司，填料含量7.7%）：预防性清洁、牙釉质成形术、用38%磷酸凝胶蚀刻咬合面、冲洗并干燥，然后在应用封闭剂之前，（1）使用釉质粘结剂（EBA，Margin Bond，德国科特威尔公司）或（2）使用DBA（Excite，列支敦士登义获嘉伟瓦登特公司）或（3）不使用粘结剂（NB）。第2组，除应用高粘度封闭剂（Seal - Rite，美国Pulpdent公司，填料含量34.4%）外，进行类似操作。标本进行热循环，然后浸入0.5%碱性品红溶液中24小时，接下来在体视显微镜下对样本的颊舌切片进行评分。采用Kruskal - Wallis检验和Mann - Whitney U检验进行数据分析。
两组中，DBA、EBA和NB亚组的微渗漏评分无显著差异。在DBA亚组（P = 0.002）和NB亚组（P = 0.041）中，低粘度封闭剂的微渗漏均低于高粘度封闭剂。
结果表明，使用低粘度封闭剂可降低窝沟封闭剂的微渗漏。然而，在放置封闭剂前使用粘结剂并不影响微渗漏。
