Yang S-Y, Kwon J-S, Kim K-N, Kim K-M
Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul, Republic of Korea BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea
J Dent Res. 2016 May;95(5):550-7. doi: 10.1177/0022034515626116. Epub 2016 Jan 14.
Enamel demineralization adjacent to pit and fissure sealants leads to the formation of marginal caries, which can necessitate the replacement of existing sealants. Dental materials with bioactive glass, which releases ions that inhibit dental caries, have been studied. The purpose of this study was to evaluate the enamel surface adjacent to sealants containing 45S5 bioactive glass (BAG) under simulated microleakage between the material and the tooth in a cariogenic environment. Sealants containing 45S5BAG filler were prepared as follows: 0% 45S5BAG + 50.0% glass (BAG0 group), 12.5% 45S5BAG + 37.5% glass (BAG12.5 group), 25.0% 45S5BAG + 25.0% glass (BAG25.0 group), 37.5% 45S5BAG + 12.5% glass (BAG37.5 group), and 50.0% 45S5BAG + 0% glass (BAG50.0 group). A cured sealant disk was placed over a flat bovine enamel disk, separated by a 60-µm gap, and immersed in lactic acid solution (pH 4.0) at 37 °C for 15, 30, and 45 d. After the storage period, each enamel disk was separated from the cured sealant disk, and the enamel surface was examined with optical 3-dimensional surface profilometer, microhardness tester, and scanning electron microscopy. The results showed a significant increase in roughness and a decrease in microhardness of the enamel surface as the proportion of 45S5BAG decreased (P< 0.05). In the scanning electron microscopy images, enamel surfaces with BAG50.0 showed a smooth surface, similar to those in the control group with distilled water, even after prolonged acid storage. Additionally, an etched pattern was observed on the surface of the demineralized enamel with a decreasing proportion of 45S5BAG. Increasing the 45S5BAG filler contents of the sealants had a significant impact in preventing the demineralization of the enamel surface within microgaps between the material and the tooth when exposed to a cariogenic environment. Therefore, despite some marginal leakage, these novel sealants may be effective preventive dental materials for inhibiting secondary caries at the margins.
窝沟封闭剂附近的牙釉质脱矿会导致边缘性龋的形成，这可能需要更换现有的封闭剂。人们已经对含有生物活性玻璃的牙科材料进行了研究，这种材料会释放抑制龋齿的离子。本研究的目的是在致龋环境中，评估含45S5生物活性玻璃（BAG）的封闭剂与牙齿之间模拟微渗漏情况下，其附近的牙釉质表面情况。含45S5BAG填料的封闭剂制备如下：0% 45S5BAG + 50.0%玻璃（BAG0组）、12.5% 45S5BAG + 37.5%玻璃（BAG12.5组）、25.0% 45S5BAG + 25.0%玻璃（BAG25.0组）、37.5% 45S5BAG + 12.5%玻璃（BAG37.5组）和50.0% 45S5BAG + 0%玻璃（BAG50.0组）。将固化后的封闭剂圆盘放置在平坦的牛牙釉质圆盘上，两者间隔60 µm的间隙，然后在37℃下浸入pH 4.0的乳酸溶液中15、30和45天。储存期结束后，将每个牙釉质圆盘与固化后的封闭剂圆盘分离，并用光学三维表面轮廓仪、显微硬度测试仪和扫描电子显微镜检查牙釉质表面。结果表明，随着45S5BAG比例的降低，牙釉质表面粗糙度显著增加，显微硬度降低（P<0.05）。在扫描电子显微镜图像中，即使经过长时间的酸储存，BAG50.0组的牙釉质表面仍显示出光滑的表面，类似于用蒸馏水处理的对照组。此外，在脱矿牙釉质表面观察到蚀刻图案，且随着45S5BAG比例的降低而愈发明显。当暴露于致龋环境时，增加封闭剂中45S5BAG填料的含量对防止材料与牙齿之间微间隙内的牙釉质表面脱矿有显著影响。因此，尽管存在一些边缘渗漏，但这些新型封闭剂可能是抑制边缘继发龋的有效预防性牙科材料。
