Zhang Zhenchao, Sang Yuhui, Wu Pucheng, Shang Yujia, Li Lesong, Duan Yujuan, Zhao Linfei, Gao Minghui, Guo Lihua, Tian Xiaowei, Yang Zhenke, Wang Shuai, Hao Lixia, Mei Xuefang
Xinxiang Key Laboratory of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453003, China.
Xinxiang Maternity and Child Healthcare Hospital, Xinxiang, Henan 453003, China.
J Trop Med. 2023 Feb 28;2023:4119956. doi: 10.1155/2023/4119956. eCollection 2023.
() could cause trichomoniasis through sexual transmission, which was globally distributed. In this study, the prevalence and phylogenetic analyses of among men in Xinxiang were conducted. From October 2018 to December 2019, a total of 634 male clinical samples were collected, including 254 samples of semen, 43 samples of prostate fluid, and 337 samples of urine. These samples were examined by nested PCR and a total of 32 (5.05%) -positive samples were detected. Among these samples, the positive rates of in semen, prostate fluid, and urine were 7.87% (20/254), 4.65% (2/43), and 2.97% (10/337), respectively. Three actin genes were successfully isolated and sequenced from the 32 positive DNA samples, and the analysis of the sequence and phylogenetic tree showed that the three actin gene sequences exhibited 99.7%-100% homology to the published actin gene sequence (EU076580) in NCBI, and the strains in the three positive samples were identified as genotype E. Our results demonstrate a notable genotype of in the male population and provide insight into the performance of these genetic markers in the molecular epidemiology of trichomoniasis. However, further studies are needed to research the association between the genotype and the pathogenicity of .
（）可通过性传播引起滴虫病，该病在全球分布。在本研究中，对新乡市男性中的（）进行了患病率及系统发育分析。2018年10月至2019年12月，共收集634份男性临床样本，包括254份精液样本、43份前列腺液样本和337份尿液样本。这些样本通过巢式PCR进行检测，共检测到32份（5.05%）阳性样本。在这些样本中，精液、前列腺液和尿液中（）的阳性率分别为7.87%（20/254）、4.65%（2/43）和2.97%（10/337）。从32份阳性DNA样本中成功分离并测序了三个肌动蛋白基因，序列及系统发育树分析表明，这三个肌动蛋白基因序列与NCBI中已发表的肌动蛋白基因序列（EU076580）具有99.7%-100%的同源性，三个阳性样本中的（）菌株被鉴定为E基因型。我们的结果证明了男性人群中（）的一种显著基因型，并为这些遗传标记在滴虫病分子流行病学中的表现提供了见解。然而，需要进一步研究以探究该基因型与（）致病性之间的关联。
