Portman O W, Alexander M
Biochim Biophys Acta. 1976 Dec 20;450(3):322-34. doi: 10.1016/0005-2760(76)90005-9.
Both low density lipoproteins and cellular membranes are known to have a high affinity for lysophosphatidylcholine. In this study lysophosphatidylcholine influenced the retention of lipoproteins by arterial tissue in vitro and the rate of disappearance of low density lipoproteins from the blood in vivo. Pieces of aorta from rabbits or rhesus monkeys were successively incubated for 90 min each in 2 or 3 solutions. After the last incubation the intima plus inner media was dissected from the remainder of the aorta for analysis. The second incubation always contained lipoproteins labeled with [3H]leucine. When lysophosphaticylcholine was included in the first but not in the second incubation fluid, the retention of low, or high density lipoproteins by the intima plus inner media increased. A subsequent incubation of the piece of artery in a fluid with trypsin or lysophosphatidylcholine caused a release of some of the lipoproteins. Lysophosphatidylcholine was bound simultaneously by plasma low density lipoproteins and vascular tissue in vitro and appeared to promote the association of the latter two components. When lysophosphatidylcholine equal to 2--10 times the usual total intravascular content was injected intravenously into control squirrel monkeys or rabbits, it was rapidly cleared from the blood. On the other hand, injected lysophosphatidylcholine persisted in the blood of hyperlipoproteinemic rabbits and was associated with the low density lipoproteins. In control animals, the injection of lysophosphatidylcholine was associated with an increase in the rate of removal of 125I-labelled low density lipoprotein from plasma and of its appearance in liver.
已知低密度脂蛋白和细胞膜对溶血磷脂酰胆碱都有很高的亲和力。在本研究中，溶血磷脂酰胆碱在体外影响动脉组织对脂蛋白的潴留，以及在体内影响低密度脂蛋白从血液中的消失速率。将兔或恒河猴的主动脉片依次在2种或3种溶液中各孵育90分钟。末次孵育后，从主动脉其余部分剥离内膜加内中膜用于分析。第二次孵育液中总是含有用[³H]亮氨酸标记的脂蛋白。当溶血磷脂酰胆碱包含在第一次而非第二次孵育液中时，内膜加内中膜对低密度或高密度脂蛋白的潴留增加。随后将动脉片在含胰蛋白酶或溶血磷脂酰胆碱的溶液中孵育会导致一些脂蛋白的释放。在体外，溶血磷脂酰胆碱同时与血浆低密度脂蛋白和血管组织结合，并且似乎促进了后两种成分的结合。当将相当于通常血管内总含量2至10倍的溶血磷脂酰胆碱静脉注射到对照松鼠猴或兔体内时，它会迅速从血液中清除。另一方面，注射的溶血磷脂酰胆碱在高脂蛋白血症兔的血液中持续存在，并与低密度脂蛋白相关。在对照动物中，注射溶血磷脂酰胆碱与从血浆中清除¹²⁵I标记的低密度脂蛋白及其在肝脏中出现的速率增加有关。
