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三种地杆菌菌株的极性脂质脂肪酸、脂多糖羟基脂肪酸和呼吸醌,以及随电子受体的变化

Polar lipid fatty acids, LPS-hydroxy fatty acids, and respiratory quinones of three Geobacter strains, and variation with electron acceptor.

作者信息

Hedrick D B, Peacock A D, Lovley D R, Woodard T L, Nevin K P, Long P E, White D C

机构信息

Microbial Insights, Inc., 2340 Stock Creek Blvd., Rockford, TN, USA.

出版信息

J Ind Microbiol Biotechnol. 2009 Feb;36(2):205-9. doi: 10.1007/s10295-008-0486-7. Epub 2008 Oct 10.

Abstract

The polar lipid fatty acids, lipopolysaccharide hydroxy-fatty acids, and respiratory quinones of Geobacter metallireducens str. GS-15, Geobacter sulfurreducens str. PCA, and Geobacter bemidjiensis str. Bem are reported. Also, the lipids of G. metallireducens were compared when grown with Fe(3+) or nitrate as electron acceptors and G. sulfurreducens with Fe(3+) or fumarate. In all experiments, the most abundant polar lipid fatty acids were 14:0, i15:0, 16:1 omega 7c, 16:1 omega 5c, and 16:0; lipopolysaccharide hydroxy-fatty acids were dominated by 3oh16:0, 3oh14:0, 9oh16:0, and 10oh16:0; and menaquinone-8 was the most abundant respiratory quinone. Some variation in lipid profiles with strain were observed, but not with electron acceptor.

摘要

报道了金属还原地杆菌菌株GS-15、硫还原地杆菌菌株PCA和贝米吉地杆菌菌株Bem的极性脂质脂肪酸、脂多糖羟基脂肪酸和呼吸醌。此外,还比较了以Fe(3+)或硝酸盐作为电子受体时生长的金属还原地杆菌的脂质,以及以Fe(3+)或富马酸作为电子受体时生长的硫还原地杆菌的脂质。在所有实验中,最丰富的极性脂质脂肪酸为14:0、异15:0、16:1 ω7c、16:1 ω5c和16:0;脂多糖羟基脂肪酸以3-OH-16:0、3-OH-14:0、9-OH-16:0和10-OH-16:0为主;甲基萘醌-8是最丰富的呼吸醌。观察到脂质谱在菌株间存在一些差异,但在电子受体间无差异。

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