Murat Dorothée, Goncalves Laurent, Dassa Elie
Unité des Membranes Bactériennes CNRS URA2172, Département de Microbiologie, Institut Pasteur 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France.
Res Microbiol. 2008 Nov-Dec;159(9-10):671-7. doi: 10.1016/j.resmic.2008.09.004. Epub 2008 Sep 20.
Bacteria use a variety of mechanisms for intercellular communication. Here we show that deletion of the uup gene, which encodes a soluble ATP binding cassette (ABC) ATPase, renders the mutant strain sensitive to its parent when they are grown together in the same medium. Our data suggest that the decrease in viability of the mutant is dependent on direct cell-to-cell contact with the parent strain. Furthermore, we show that the presence of intact Walker B motifs in Uup is required for immunity or resistance to the parental strain, suggesting that ATP hydrolysis is an important determinant of this phenotype.
细菌利用多种机制进行细胞间通讯。在此我们表明，编码可溶性ATP结合盒（ABC）ATP酶的uup基因缺失后，当突变菌株与亲本菌株在同一培养基中共同培养时，突变菌株对亲本敏感。我们的数据表明，突变体活力的降低取决于与亲本菌株的直接细胞间接触。此外，我们表明Uup中完整的沃克B基序的存在是对亲本菌株免疫或抗性所必需的，这表明ATP水解是该表型的一个重要决定因素。
