Niederländer Harm A G, van Beek Teris A, Bartasiute Aiste, Koleva Irina I
Solvay Pharmaceuticals BV, Pharmaceutical & Analytical Development, C.J. van Houtenlaan 36 (WNH-117), 1381 CP Weesp, The Netherlands.
J Chromatogr A. 2008 Nov 14;1210(2):121-34. doi: 10.1016/j.chroma.2008.09.061. Epub 2008 Sep 24.
Screening for antioxidants requires simple in vitro model systems to investigate antioxidant activity. High resolution screening (HRS), combining a separation technique like HPLC with fast post-column (bio)chemical detection can rapidly pinpoint active compounds in complex mixtures. In this paper both electrochemical and chemistry-based assays are reviewed and discussed. The focus is on the mechanisms involved and differences between the assays, rather than on the matrix or analytes. With 45 applications high resolution antioxidant screening has now become an almost routine tool for the rapid identification of antioxidants in plant extracts, foods and beverages. The methods based on true reactive oxygen species (ROS) provide the most realistic measure of antioxidant activity. Unfortunately these methods are difficult to set up and control and have not been applied since they were reported. The methods based on electrochemical detection are more practical, but have still received only limited attention for practical screening purposes. The methods based on a single relatively stable reagent such as DPPH and ABTS(+) have become most popular, because of their simple set-up and ease of control. The methods have been combined with on-line DAD, MS and NMR detection for rapid identification of active constituents.
抗氧化剂的筛选需要简单的体外模型系统来研究抗氧化活性。高分辨率筛选（HRS）将高效液相色谱（HPLC）等分离技术与快速柱后（生物）化学检测相结合，能够快速确定复杂混合物中的活性化合物。本文对抗电化学和基于化学的分析方法进行了综述和讨论。重点在于所涉及的机制以及分析方法之间的差异，而非基质或分析物。凭借45个应用实例，高分辨率抗氧化剂筛选现已成为快速鉴定植物提取物、食品和饮料中抗氧化剂的常用工具。基于真正活性氧（ROS）的方法提供了最实际的抗氧化活性测量。不幸的是，这些方法难以建立和控制，自报道以来尚未得到应用。基于电化学检测的方法更具实用性，但在实际筛选中仍未得到足够关注。基于单一相对稳定试剂（如DPPH和ABTS(+)）的方法最为流行，因其设置简单且易于控制。这些方法已与在线二极管阵列检测器（DAD）、质谱（MS）和核磁共振（NMR）检测相结合，用于快速鉴定活性成分。
