Qingdao Key Laboratory of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, SOA 266061, Qingdao, China.
Nat Prod Res. 2012;26(9):873-7. doi: 10.1080/14786419.2011.565285. Epub 2011 Oct 14.
A method based on high-performance liquid chromatography (HPLC) with diode array detector coupled with electrospray ionisation-mass spectrometry and an online detection system for radical scavenging was established and used to rapidly find and quantify antioxidant compounds in the water extracts of Hippocampus japonicus Kaup, Hippocampus kuda Bleeker and Syngnathus acus Linnaeus. The online screening results revealed the presence of one major radical scavenging compound identified as hypoxanthine by comparison of mass data and retention time with the standard. Subsequently, the developed HPLC method was applied to quantify hypoxanthine in different H. japonicus, H. kuda and S. acus samples. The results indicated that the developed HPLC method is simple and reliable for the quantification of hypoxanthine with a detection limit at 0.002 µg mL(-1), and a high recovery from 96.3% to 102.1%. This method provides a powerful tool for rapid identification and quantification of free radical scavenging compounds in complex marine natural products.
建立了一种基于高效液相色谱（HPLC）与二极管阵列检测器联用的方法，并结合电喷雾离子化-质谱和在线检测系统，用于快速发现和定量海马、海鳗和尖海龙水提取物中的抗氧化化合物。在线筛选结果表明，通过与标准品的质谱数据和保留时间比较，存在一种主要的自由基清除化合物，鉴定为次黄嘌呤。随后，将开发的 HPLC 方法应用于不同海马、海鳗和尖海龙样品中次黄嘌呤的定量。结果表明，该 HPLC 方法简单可靠，用于次黄嘌呤的定量检测，检测限为 0.002µg/mL，回收率为 96.3%至 102.1%。该方法为快速鉴定和定量复杂海洋天然产物中的自由基清除化合物提供了有力工具。
