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采用体外试验和在线筛选HPLC-ABTS系统从黄芩中提取主要黄酮类化合物并进行生物活性分析

Extraction and Bioactivity Analysis of Major Flavones Compounds from Scutellaria baicalensis Using In Vitro Assay and Online Screening HPLC-ABTS System.

作者信息

Lee Kwang Jin, Jung Pil Mun, Oh You-Chang, Song Na-Young, Kim Taesoo, Ma Jin Yeul

机构信息

KM-Based Herbal Drug Development Group, Korean Institute of Oriental Medicine (KIOM), 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea.

出版信息

J Anal Methods Chem. 2014;2014:563702. doi: 10.1155/2014/563702. Epub 2014 Sep 1.

DOI:10.1155/2014/563702
PMID:25258697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4166446/
Abstract

The extraction efficiency of a number of solvent compositions for the improvement of bioactive compounds yield from S. baicalensis has been investigated. Also, free radical scavengers in the glycoside baicalin (BG), wogonoside (WG), aglycon baicalein (B), and wogonin (W) compounds of S. baicalensis were screened, identified, and quantified using coupled offline ABTS and online screening HPLC-ABTS assay. Increasing ethanol content fractions resulted in decreased extract yield of bioactive compounds. In this case, the best yield of 37.01 mg/g in BG, WG, B, and W compounds was obtained by a dipping method with an extraction time of 4 h. In addition, the yield (43.05%) and IC50 (34.04 μg/mL) determined through ABTS assay of the 60% aqueous ethanol extract were the most satisfactory of all solvent solutions tested. This result shows that an online screening HPLC-ABTS assay can be a powerful technique for the rapid characterization of bioactivity compounds in plant extracts. Moreover, their anti-inflammatory activities were evaluated via analyzed inhibitory effect on NO and inflammatory cytokine production. Furthermore, WG and W exhibited the strong inhibitory effects on inflammatory mediator production including NO, IL-6, and IL-1β in LPS-stimulated RAW 264.7 macrophages.

摘要

研究了多种溶剂组合物对提高黄芩生物活性化合物产量的提取效率。此外，还使用离线ABTS与在线筛选HPLC-ABTS联用分析法对黄芩中的糖苷黄芩苷（BG）、汉黄芩苷（WG）、苷元黄芩素（B）和汉黄芩素（W）化合物中的自由基清除剂进行了筛选、鉴定和定量。乙醇含量分数增加导致生物活性化合物的提取物产量降低。在这种情况下，通过浸渍法在4小时的提取时间下，BG、WG、B和W化合物的最佳产量为37.01mg/g。此外，通过ABTS分析法测定的60%乙醇水溶液提取物的产率（43.05%）和IC50（34.04μg/mL）在所有测试的溶剂溶液中是最令人满意的。该结果表明，在线筛选HPLC-ABTS分析法可以成为快速表征植物提取物中生物活性化合物的有力技术。此外，通过分析对一氧化氮（NO）和炎性细胞因子产生的抑制作用来评估它们的抗炎活性。此外，WG和W对脂多糖刺激的RAW 264.7巨噬细胞中包括NO、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）在内的炎性介质产生具有强烈的抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/e89b9d64fe2b/JAMC2014-563702.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/cea1cec9deaf/JAMC2014-563702.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/c5ee89d933cf/JAMC2014-563702.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/9123c79cd076/JAMC2014-563702.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/07b14babe89f/JAMC2014-563702.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/2509f50edce5/JAMC2014-563702.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/c1b98928f40e/JAMC2014-563702.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/e89b9d64fe2b/JAMC2014-563702.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/cea1cec9deaf/JAMC2014-563702.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/c5ee89d933cf/JAMC2014-563702.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/9123c79cd076/JAMC2014-563702.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/07b14babe89f/JAMC2014-563702.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/2509f50edce5/JAMC2014-563702.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/c1b98928f40e/JAMC2014-563702.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/91e9/4166446/e89b9d64fe2b/JAMC2014-563702.007.jpg

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