Laboratory of Biochemistry, National Institute for Digestive Diseases, Via della Resistenza, 70013, Castellana Grotte (Bari), Italy.
Genes Nutr. 2008 Apr;3(1):35-40. doi: 10.1007/s12263-008-0082-5.
The major soy-derived isoflavones such as genistein has been demonstrated to possess anticarcinogenic activity in animal model systems. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 to 50 muM on the LDL receptor and HMG-CoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMG-CoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an increase of LDL receptor gene expression and later decrease of HMG-CoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMG-CoA reductase gene expression in colon cancer.
主要的大豆异黄酮，如染料木黄酮，已被证明在动物模型系统中具有抗癌活性。本研究旨在探讨浓度范围为 0.01 至 50 μM 的异黄酮染料木黄酮暴露对雌激素受体阳性 DLD-1 人结肠癌细胞系中 LDL 受体和 HMG-CoA 还原酶基因表达的影响。通过反转录实时 PCR 评估 LDL 受体和 HMG-CoA 还原酶基因的表达。染料木黄酮诱导 DLD-1 细胞中 LDL 受体基因表达增加，随后 HMG-CoA 还原酶 mRNA 表达降低。这些发现为染料木黄酮在调节结肠癌中 LDL 受体和 HMG-CoA 还原酶基因表达中的作用提供了直接证据。
