Department of Dental Pharmacology, School of Dentistry, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
Genes Nutr. 2008 Feb;2(4):371-4. doi: 10.1007/s12263-007-0072-z.
It has been reported that the gum resin of Boswellia serrata (BS), which has been shown to have antiinflammatory properties, might also have anticancer effects. This study examined the potential of BS as an anticancer agent. The BS extract induces apoptosis in HeLa human cervical carcinoma cells, as confirmed by two apoptosis analyses, Hoechst staining and Annexin V/PI assay. Among the apoptosis pathways, the ER stress-associated mechanism was examined to determine its role in BS-induced apoptosis. The expression of GRP78 and CHOP, which are representatives of the ER stress proteins, and the calcium-binding protein-calpain were determined. The results showed significantly higher levels of both GRP78 and CHOP, and stronger calpain activity in the BS-treated cells than in the control cells. This shows that there is a correlation between ER stress signaling and apoptosis, which suggests the possibility of the BS-ER stress initiator as an anticancer therapeutic agent in human cervical carcinoma.
据报道，具有抗炎特性的 Boswellia serrata（乳香）树胶可能也具有抗癌作用。本研究探讨了乳香作为抗癌剂的潜力。乳香提取物通过两种凋亡分析、Hoechst 染色和 Annexin V/PI 检测证实，可诱导 HeLa 人宫颈癌细胞凋亡。在凋亡途径中，检测了与 ER 应激相关的机制，以确定其在 BS 诱导的凋亡中的作用。测定了 ER 应激蛋白代表物 GRP78 和 CHOP 以及钙结合蛋白-calpain 的表达。结果显示，BS 处理的细胞中 GRP78 和 CHOP 的表达水平以及 calpain 活性均明显高于对照组。这表明 ER 应激信号与凋亡之间存在相关性，这表明 BS-ER 应激启动子作为人类宫颈癌的抗癌治疗剂的可能性。
