Nagaraj P, Aradhana N, Shivakumar Anantharaman, Shrestha Ashwinee Kumar, Gowda Avinash K
Department of Studies in Chemistry, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka State, India.
Environ Monit Assess. 2009 Oct;157(1-4):575-82. doi: 10.1007/s10661-008-0557-2. Epub 2008 Oct 11.
A simple and sensitive spectrophotometric method for the determination of chromium has been developed. The method is based on the diazotization of Dapsone in hydroxylamine hydrochloride medium and coupling with N-(1-Napthyl) Ethylene Diamine Dihydrochloride by electrophilic substitution to produce an intense pink azo-dye, which has absorption maximum at 540 nm. The Beer's law is obeyed from 0.02-1.0 microg mL(-1) and the molar absorptivity is 3.4854 L mol(-1) cm(-1). The Limits of quantification and Limit of detection of the proposed method are 0.0012 microg mL(-1) and 0.0039 microg mL(-1) respectively. The method has been successfully applied for the determination of chromium in water samples and the results were statistically evaluated with that of the reference method.
已开发出一种简单灵敏的分光光度法用于测定铬。该方法基于在盐酸羟胺介质中对氨苯砜进行重氮化，并通过亲电取代与盐酸N-(1-萘基)乙二胺偶联，生成一种强烈的粉红色偶氮染料，其在540nm处有最大吸收。在0.02 - 1.0μg mL(-1)范围内符合比尔定律，摩尔吸光系数为3.4854 L mol(-1) cm(-1)。该方法的定量限和检测限分别为0.0012μg mL(-1)和0.0039μg mL(-1)。该方法已成功应用于水样中铬的测定，并与参考方法的结果进行了统计学评估。
