Revanasiddappa H D, Kiran Kumar T N
Department of Chemistry, University of Mysore, Manasagangothri, Mysore 570 006, India.
Talanta. 2003 May 28;60(1):1-8. doi: 10.1016/S0039-9140(02)00567-2.
A highly sensitive and selective spectrophotometric method was developed for the determination of trace amounts of chromium with leuco Xylene cynaol FF. The method is based on the oxidation of leuco Xylene cyanol FF (LXCFF) to its blue form of Xylene cyanol FF by chromium(VI) in sulphuric acid medium (pH 1.2-2.4), the absorbance of the formed dye is measured in an acetate buffer medium (pH 3.0-4.6) at 615 nm. The method obeys Beer's law in the concentration range of 0.05-0.45 mug ml(-l) chromium, having molar absorptivity and Sandell's sensitivity of 8.23x10(4) l mol(-1) cm(-l) and 0.00063 mug cm(-2), respectively. All the variables were studied in order to optimise the reaction conditions. The developed method has been successfully applied to the determination of chromium in steels, pharmaceutical samples, industrial effluents, natural water, and soil.
开发了一种高灵敏度和高选择性的分光光度法，用于用无色二甲苯蓝FF测定痕量铬。该方法基于在硫酸介质（pH 1.2 - 2.4）中，铬（VI）将无色二甲苯蓝FF（LXCFF）氧化为其蓝色形式的二甲苯蓝FF，在醋酸盐缓冲介质（pH 3.0 - 4.6）中于615 nm处测量形成染料的吸光度。该方法在铬浓度范围为0.05 - 0.45 μg ml⁻¹时符合比尔定律，摩尔吸光率和桑德尔灵敏度分别为8.23×10⁴ l mol⁻¹ cm⁻¹和0.00063 μg cm⁻²。研究了所有变量以优化反应条件。所开发的方法已成功应用于钢、药物样品、工业废水、天然水和土壤中铬的测定。
