Müller K, Diamant M, Bendtzen K
Laboratory for Medical Immunology, Rigshospitalet (State University Hospital), Copenhagen, Denmark.
Immunol Lett. 1991 May;28(2):115-20. doi: 10.1016/0165-2478(91)90108-m.
1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3) has been shown to interfere with immunoglobulin production and lymphocyte proliferation in vitro. These lymphocyte functions are influenced by interleukin (IL)-6 produced by antigen presenting cells. Hence, the ability of 1,25-(OH)2D3 to interfere with the production and function of IL-6 was investigated. 1,25-(OH)2D3 and the analogue MC 903 inhibited IL-6 production by LPS-stimulated human mononuclear cells. The precursor 25-OH D3 was ineffective. Likewise, 1,25-(OH)2D3 but not 25-OH D3 inhibited rIL-6-driven as well as rIL-1 alpha/beta-driven proliferation of murine thymocytes. This effect of 1,25-(OH)2D3 was partially or totally overcome by larger concentrations of rIL-6 as well as by rIL-2 and ionomycin. Consistently, the production of IL-6 and IL-2 in rIL-1 driven thymocyte cultures were found to be reduced by 1,25-(OH)2D3. Inhibition of production and function of IL-6 may therefore be involved in 1,25-(OH)2D3-mediated regulation of lymphocyte functions in vitro.
1,25-二羟基维生素D3（1,25-(OH)2D3）已被证明在体外会干扰免疫球蛋白的产生和淋巴细胞增殖。这些淋巴细胞功能受抗原呈递细胞产生的白细胞介素（IL）-6影响。因此，研究了1,25-(OH)2D3干扰IL-6产生和功能的能力。1,25-(OH)2D3及其类似物MC 903抑制脂多糖刺激的人单核细胞产生IL-6。前体25-OH D3无此作用。同样，1,25-(OH)2D3而非25-OH D3抑制重组IL-6驱动的以及重组IL-1α/β驱动的小鼠胸腺细胞增殖。更高浓度的重组IL-6以及重组IL-2和离子霉素可部分或完全克服1,25-(OH)2D3的这一作用。一致地，在重组IL-1驱动的胸腺细胞培养物中，发现1,25-(OH)2D3可降低IL-6和IL-2的产生。因此，抑制IL-6的产生和功能可能参与了1,25-(OH)2D3在体外介导的淋巴细胞功能调节。
