Smith J, Jenkins A S, Caine S, Boyle I T
University Department of Medicine, Glasgow Royal Infirmary, Scotland, UK.
J Clin Lab Immunol. 1990 Oct;33(2):49-54.
Peripheral blood mononuclear cells (PBMC) display receptors for parathyroid hormone (PTH) and calcitonin (CT) and, when activated, express receptors for 1,25 dihydroxyvitamin D3 (1,25(OH)2D3). The role of these receptors in unclear. It is well established that 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) has a significant effect on lymphocyte transformation and interleukin-2 (IL-2) activity in PBMC's. We have proceeded to compare the effects of the calciotrophic hormones, 1,25(OH)2D3, 24,25 dihydroxyvitamin D3 (24,25(OH)2D3), 25 dihydroxyvitamin D3 (25(OH)D3), bovine (1-35)PTH (b(1-35)PTH) and salmon CT on both lymphocyte transformation and IL-2 activity in PBMC's from normal human volunteers. We have also sought an indication as to which subset of cells is responsible for the 1,25(OH)2D3 effect. Unlike the other calciotrophic hormones 1,25(OH)2D3 inhibited the proliferation of phytohemagglutinin (PHA), concanavalin A (ConA) and pokeweed mitogen (PWM) stimulated cells. 1,25(OH)2D3 had a significantly greater effect on PHA induced cell proliferation and only inhibited cells with the T-helper/inducer (TH, CD4 + ve) phenotype, this suggested that 1,25(OH)2D3 may act selectively on the cells with CD4 + ve phenotype. In addition, of the calciotrophic hormones, 1,25(OH)2D3 had a greater effect on IL-2 activity. These findings indicate that 1,25(OH)2D3 and not the other calciotrophic hormones has a significant immunomodulatory effect.
外周血单个核细胞（PBMC）表达甲状旁腺激素（PTH）和降钙素（CT）的受体，激活后还表达1,25-二羟维生素D3（1,25(OH)2D3）的受体。这些受体的作用尚不清楚。众所周知，1,25-二羟维生素D3（1,25(OH)2D3）对PBMC中的淋巴细胞转化和白细胞介素-2（IL-2）活性有显著影响。我们进一步比较了钙调节激素1,25(OH)2D3、24,25-二羟维生素D3（24,25(OH)2D3）、25-羟维生素D3（25(OH)D3）、牛（1-35）PTH（b(1-35)PTH）和鲑鱼CT对正常人类志愿者PBMC中淋巴细胞转化和IL-2活性的影响。我们还试图确定哪种细胞亚群对1,25(OH)2D3的作用负责。与其他钙调节激素不同，1,25(OH)2D3抑制植物血凝素（PHA）、刀豆球蛋白A（ConA）和商陆有丝分裂原（PWM）刺激的细胞增殖。1,25(OH)2D3对PHA诱导的细胞增殖影响更大，且仅抑制具有T辅助/诱导（TH，CD4 + 阳性）表型的细胞，这表明1,25(OH)2D3可能选择性地作用于具有CD4 + 阳性表型的细胞。此外，在钙调节激素中，1,25(OH)2D3对IL-2活性的影响更大。这些发现表明，具有显著免疫调节作用的是1,25(OH)2D3而非其他钙调节激素。
