Sniderman A D, Cianflone K M, Eckel R H
McGill Unit for the Prevention of Cardiovascular Disease, McGill University, Montreal, Quebec, Canada.
Int J Obes. 1991 May;15(5):333-6.
Acylation stimulating protein (ASP) is a small (MW 14,000) basic (pI 9.0) protein which has only recently been purified from human plasma. Since ASP is the most potent known stimulant of triglyceride synthesis in human adipose tissue, the present study was designed to determine if plasma ASP was elevated in patients with moderate obesity, and if so, whether this level changed with weight loss. Fasting plasma ASP levels were determined by competitive ELISA immunoassay in 10 obese women before weight loss, immediately after weight loss, and 3 months after maintaining weight reduction. Their plasma ASP results were compared to 17 age and sex-matched lean controls. With weight loss, plasma ASP decreased significantly: 19.6 +/- 10.7 mg/dl before weight loss vs 15.0 +/- 9.5 mg/dl after weight loss vs 13.8 +/- 7.7 mg/dl 3 months after being weight stable, P less than 0.05 initial vs final value. Nevertheless, plasma ASP was significantly higher than the control value at all three times. Thus, before weight loss, the average ASP in the obese group was four times that in the control group (19.6 +/- 10.7 vs 5.1 +/- 3.6 mg/dl, P less than 0.0005) while even 3 months after weight loss, it remained almost three times above the control group (13.8 +/- 7.7 vs 5.1 +/- 3.6 mg/dl, P less than 0.0005). The data suggest, therefore, that an elevated plasma level of ASP is common in obesity, that the level of plasma ASP may reflect the fat cell mass present in an individual, and raises the possibilities that ASP may play a role in initiation or maintenance of the obese state.
酰化刺激蛋白（ASP）是一种分子量较小（14,000）的碱性蛋白（pH值9.0），直到最近才从人血浆中纯化出来。由于ASP是已知的人类脂肪组织中甘油三酯合成最有效的刺激物，本研究旨在确定中度肥胖患者的血浆ASP水平是否升高，如果升高，那么该水平是否会随着体重减轻而变化。通过竞争性酶联免疫吸附测定法，测定了10名肥胖女性在减肥前、减肥后即刻以及体重减轻维持3个月后的空腹血浆ASP水平。将她们的血浆ASP检测结果与17名年龄和性别匹配的瘦人对照组进行比较。随着体重减轻，血浆ASP显著降低：减肥前为19.6±10.7mg/dl，减肥后为15.0±9.5mg/dl，体重稳定3个月后为13.8±7.7mg/dl，初始值与最终值相比P<0.05。然而，在所有三个时间点，血浆ASP均显著高于对照组值。因此，减肥前，肥胖组的平均ASP是对照组的四倍（19.6±10.7对5.1±3.6mg/dl，P<0.0005），而即使在减肥3个月后，其仍比对照组高出近三倍（13.8±7.7对5.1±3.6mg/dl，P<0.0005）。因此，数据表明，肥胖患者血浆ASP水平升高很常见，血浆ASP水平可能反映个体中存在的脂肪细胞量，并增加了ASP可能在肥胖状态的发生或维持中起作用的可能性。
