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ROCK1和LIMK2在铺展型而非气泡型癌细胞中相互作用。

ROCK1 and LIMK2 interact in spread but not blebbing cancer cells.

作者信息

Shea Kerry F, Wells Claire M, Garner Andrew P, Jones Gareth E

机构信息

Randall Division of Cell & Molecular Biophysics, King's College London, London, United Kingdom.

出版信息

PLoS One. 2008;3(10):e3398. doi: 10.1371/journal.pone.0003398. Epub 2008 Oct 14.

DOI:10.1371/journal.pone.0003398
PMID:18852895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2561063/
Abstract

Cancer cells migrating within a 3D microenvironment are able to adopt either a mesenchymal or amoeboid mode of migration. Amoeboid migration is characterised by membrane blebbing that is dependent on the Rho effectors, ROCK1/2. We identify LIMK2 as the preferred substrate for ROCK1 but find that LIMK2 did not induce membrane blebbing, suggesting that a LIMK2 pathway is not involved in amoeboid-mode migration. In support of this hypothesis, novel FRET data demonstrate a direct interaction between ROCK1 and LIMK2 in polarised but not blebbing cells. Our results point to a specific role for the ROCK1:LIMK2 pathway in mesenchymal-mode migration.

摘要

在三维微环境中迁移的癌细胞能够采用间充质或阿米巴样迁移模式。阿米巴样迁移的特征是膜泡化，这依赖于Rho效应器ROCK1/2。我们确定LIMK2是ROCK1的首选底物，但发现LIMK2不会诱导膜泡化，这表明LIMK2途径不参与阿米巴样迁移模式。为支持这一假设，新的荧光共振能量转移数据表明，ROCK1和LIMK2在极化而非膜泡化的细胞中存在直接相互作用。我们的结果表明ROCK1:LIMK2途径在间充质迁移模式中具有特定作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/8a5e57545de1/pone.0003398.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/f463758ad37a/pone.0003398.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/7246ce9d260d/pone.0003398.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/aeeb3008f82b/pone.0003398.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/8a5e57545de1/pone.0003398.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/f463758ad37a/pone.0003398.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/7246ce9d260d/pone.0003398.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/aeeb3008f82b/pone.0003398.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79e8/2561063/8a5e57545de1/pone.0003398.g004.jpg

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