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[用于检测气单胞菌属和邻单胞菌属的新型选择性指示培养基:UNISC琼脂]

[New selective indicator medium for detection of Aeromonas and Plesiomonas: UNISC agar].

作者信息

Rocha Marion Pereira da, Scheid Liliane A, Alves Sydney Hartz

机构信息

Universidade de Santa Cruz, Santa Cruz do Sul, RS, Brasil.

出版信息

Rev Soc Bras Med Trop. 2008 Jul-Aug;41(4):431-3. doi: 10.1590/s0037-86822008000400024.

Abstract

We evaluated a new selective indicator medium (UNISC Agar) for isolation of classical enteropathogens, Aeromonas spp and Plesiomonas shigelloides. The xylose fermentation capacity is indicated by a yellow color (fermenting agents) or blue (no fermenting agent). This, together with the oxidase test, establishes it as an indicator for detecting Aeromonas and Plesiomonas shigelloides. Its productivity and selectivity, as assessed using the absolute count index and relative count index, indicate it as an alternative to the classical feces culturing media. This is because, in a single medium, it enables isolation of Escherichia coli, Shigella spp and Salmonella spp, in addition to Aeromonas and Plesiomonas shigelloides, thereby favoring the laboratory diagnosis of gastroenteritis.

摘要

我们评估了一种用于分离经典肠道病原体、气单胞菌属和类志贺邻单胞菌的新型选择性指示培养基(UNISC琼脂)。木糖发酵能力通过黄色(发酵剂)或蓝色(无发酵剂)表示。这与氧化酶试验一起,使其成为检测气单胞菌和类志贺邻单胞菌的指标。使用绝对计数指数和相对计数指数评估其生产力和选择性,表明它可替代传统的粪便培养基。这是因为在单一培养基中,它除了能分离出气单胞菌和类志贺邻单胞菌外,还能分离出大肠杆菌、志贺氏菌属和沙门氏菌属,从而有利于肠胃炎的实验室诊断。

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