Barrett D A, Shaw P N, Davis S S
Department of Pharmaceutical Sciences, University Park, University of Nottingham, U.K.
J Chromatogr. 1991 May 3;566(1):135-45. doi: 10.1016/0378-4347(91)80118-v.
A method is described for the simultaneous determination of morphine and 6-acetylmorphine in small volumes of human plasma by normal-phase high-performance liquid chromatography using solid-phase extraction, dansyl derivatisation and fluorescence detection. The lower limits of quantitation in a 0.1-ml plasma sample are 10 ng/ml for morphine and 25 ng/ml for 6-acetylmorphine. The method has been applied to determine concentrations of morphine and 6-acetylmorphine in plasma samples from premature babies administered an intravenous infusion of diamorphine.
描述了一种通过正相高效液相色谱法同时测定少量人血浆中吗啡和6-乙酰吗啡的方法，该方法采用固相萃取、丹磺酰衍生化和荧光检测。在0.1 ml血浆样品中，吗啡的定量下限为10 ng/ml，6-乙酰吗啡的定量下限为25 ng/ml。该方法已应用于测定接受静脉注射二醋吗啡的早产儿血浆样品中吗啡和6-乙酰吗啡的浓度。
