Rop P P, Grimaldi F, Burle J, De Saint Leger M N, Viala A
Laboratoire Interrégional de Police Scientifique, Marseille, France.
J Chromatogr B Biomed Appl. 1994 Nov 18;661(2):245-53. doi: 10.1016/0378-4347(94)00345-9.
6-Monoacetylmorphine and morphine were determined simultaneously in plasma, whole blood and urine, after solid-phase extraction, by high-performance liquid chromatography using amperometric detection at 600 mV oxidation potential. The recoveries ranged from 92 to 99%. The reproducibility study indicated that the coefficients of variation were less than 11% for morphine and 12.4% for 6-monoacetylmorphine. The determination limits were 1 ng/ml for morphine and 4 ng/ml for 6-monoacetylmorphine. The method had a good selectivity towards opiate and nonopiate analgesics and other drugs. The stability of the analytes in methanol (standard solutions), in samples (plasma, whole blood and urine) at -20 degrees C and at 20 degrees C, and in samples after enzymatic hydrolysis at 37 degrees C, was also studied. For sample containing 6-monoacetylmorphine, inadequate storage or hydrolysis could lead to overestimation of morphine or its conjugates. The technique described can be applied for the study of the pharmacokinetics of heroin; it is also available for forensic toxicology to distinguish heroin use from medical prescription of morphine and other opiate drugs.
采用高效液相色谱法，在600 mV氧化电位下进行安培检测，在固相萃取后同时测定血浆、全血和尿液中的6-单乙酰吗啡和吗啡。回收率在92%至99%之间。重现性研究表明，吗啡的变异系数小于11%，6-单乙酰吗啡的变异系数为12.4%。吗啡的测定限为1 ng/ml，6-单乙酰吗啡的测定限为4 ng/ml。该方法对阿片类和非阿片类镇痛药及其他药物具有良好的选择性。还研究了分析物在甲醇（标准溶液）、-20℃和20℃下的样品（血浆、全血和尿液）以及37℃酶水解后的样品中的稳定性。对于含有6-单乙酰吗啡的样品，储存或水解不当可能导致吗啡或其共轭物的高估。所描述的技术可用于海洛因的药代动力学研究；它也可用于法医毒理学，以区分海洛因的使用与吗啡和其他阿片类药物的医疗处方。
