van de Water C, Haagsma N
Faculty of Veterinary Medicine, Department of the Science of Food and Animal Origin, University of Utrecht, The Netherlands.
J Chromatogr. 1991 May 3;566(1):173-85. doi: 10.1016/0378-4347(91)80122-s.
Both screening and quantitative methods for chloramphenicol residues in swine tissues and milk were compared, using samples from animals treated with chloramphenicol. For screening purposes a previously developed streptavidin-biotin enzyme-linked immunosorbent assay and a commercially available immunochemical card test were used. For quantitative purposes two previously developed high-performance liquid chromatographic procedures were applied using antibody-mediated clean-up and solid-phase extraction. Some improvements in both methods were also described. The results obtained with the screening tests and those obtained with the quantitative methods correspond well with each other. Using a combination of these methods, an effective control of residues of chloramphenicol can be performed in milk from the 1 microgram/kg level and in swine tissues from the 10 micrograms/kg level.
采用来自用氯霉素治疗过的动物的样本，对猪组织和牛奶中氯霉素残留的筛查和定量方法进行了比较。为进行筛查，使用了一种先前开发的链霉亲和素-生物素酶联免疫吸附测定法以及一种市售的免疫化学卡片检测法。为进行定量，应用了两种先前开发的高效液相色谱程序，采用抗体介导的净化和固相萃取。还描述了两种方法的一些改进之处。筛查试验得到的结果与定量方法得到的结果相互吻合良好。结合使用这些方法，可以对牛奶中1微克/千克水平及猪组织中10微克/千克水平的氯霉素残留进行有效控制。
