van de Water C, Haagsma N, van Kooten P J, van Eden W
Department of the Science of Food of Animal Origin, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Z Lebensm Unters Forsch. 1987 Sep;185(3):202-7. doi: 10.1007/BF01042047.
A competitive enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody is described for the determination of chloramphenicol (CAP) residues in swine muscle tissue. The limit of detection of standard solutions is established to be 25 ng ml-1 = 2.0 ng CAP per well). The very high specificity of the monoclonal antibody for CAP is expressed by the insignificant cross-reactivity with other antimicrobial agents and with structurally related compounds. By means of the rapid sample preparation method described earlier for the CAP determination using high-performance liquid chromatography (HPLC), residue levels of CAP in swine muscle tissue above 5 micrograms kg-1 can be easily quantitated. The muscle samples show good recovery percentages at 10-50 micrograms kg-1 spiking levels. The results obtained by the ELISA method were confirmed by HPLC.
描述了一种使用单克隆抗体的竞争性酶联免疫吸附测定（ELISA）法，用于测定猪肌肉组织中的氯霉素（CAP）残留。标准溶液的检测限确定为25 ng/ml（每孔2.0 ng CAP）。单克隆抗体对CAP具有非常高的特异性，与其他抗菌剂和结构相关化合物的交叉反应不明显。通过先前描述的用于使用高效液相色谱（HPLC）测定CAP的快速样品制备方法，可以轻松定量猪肌肉组织中高于5微克/千克的CAP残留水平。肌肉样品在10 - 50微克/千克的加标水平下显示出良好的回收率。ELISA法获得的结果通过HPLC得到了证实。
