Baillou A, Janvier B, Leonard G, Denis F, Goudeau A, Barin F
Unité de Virologie, département de Microbiologie médicale et moléculaire, Unité de Recherche Associée, 1334 Centre National de la Recherche Scientifique, CHRU Bretonneau, Tours Cedex, France.
J Clin Microbiol. 1991 Jul;29(7):1387-91. doi: 10.1128/jcm.29.7.1387-1391.1991.
In this study, enzyme immunoassays for detection of type-specific antibodies to human immunodeficiency viruses (HIV) were developed by using short peptides corresponding to sequences located within the immunodominant domain of the transmembrane glycoproteins of both HIV-1 and HIV-2-simian immunodeficiency virus (SIV). The assays were highly sensitive with currently available sera from various geographical areas. Furthermore, they appeared to be more specific in HIV serotyping than the Western blot (immunoblot) assay, since all of the sera were clearly discriminated as one or the other type. It was also shown that in contrast to HIV-1, the C-terminal cysteine residue (amino acid 620, SIV from captive macaques, Mm142 strain) of the HIV-2-SIV peptide is not necessary for recognition of the peptide by antibody to HIV-2.
在本研究中，通过使用与HIV-1和HIV-2-猴免疫缺陷病毒（SIV）跨膜糖蛋白免疫显性区域内序列相对应的短肽，开发了用于检测人免疫缺陷病毒（HIV）型特异性抗体的酶免疫测定法。这些测定法对来自不同地理区域的现有血清具有高度敏感性。此外，它们在HIV血清分型中似乎比蛋白质印迹（免疫印迹）测定法更具特异性，因为所有血清都能清晰地被区分为这一型或那一型。还表明，与HIV-1相反，HIV-2-SIV肽的C末端半胱氨酸残基（来自圈养猕猴的SIV，Mm142株的氨基酸620）对于HIV-2抗体识别该肽不是必需的。
