Sprecher D L, Knauer S L, Black D M, Kaplan L A, Akeson A A, Dusing M, Lattier D, Stein E A, Rymaszewski M, Wiginton D A
Lipid Research Clinic, General Clinical Research Center, University of Cincinnati, Ohio 45267.
J Clin Invest. 1991 Sep;88(3):985-94. doi: 10.1172/JCI115402.
Our primary aim was to determine the extent to which intraplasmic retinyl palmitate (RP) transfers to other lipoprotein particles when chylomicron remnants are not produced and/or the plasma RP residence time is increased. The study was conducted on three familial type I hyperlipoproteinemic patients, four lipoprotein lipase (LpL)-deficient heterozygotes, and three controls on a metabolic research unit. To each subject, a fat load was administered containing 16% of total daily calories in type I patients, 40% in heterozygotes and controls, plus 60,000 U/m2 vitamin A. Triglyceride (TG) and RP levels were evaluated in chylomicron and nonchylomicron fractions. Delay in the clearance of chylomicron fraction RP and the marked deficiency in nonchylomicron-RP (presumed lack of remnant production) in all three type I patients suggests that RP does not demonstrate significant intraplasmic transfer from chylomicrons to existent apolipoprotein B100 particles. In contrast to noncoincident TG and RP peaking in the normal subject, heterozygotes were found to demonstrate coincident plasma TG and RP curves, which is consistent with a common catabolic pathway for both TG and RP and inconsistent with intraplasmic RP transfer. This corroborates reports on compromised chylomicron clearance in heterozygotes. We conclude that RP is an appropriate representative marker for intestinally derived particles in LpL-deficient or partially deficient individuals.
我们的主要目的是确定在不产生乳糜微粒残粒和/或血浆视黄醇棕榈酸酯（RP）停留时间增加的情况下，胞质内视黄醇棕榈酸酯（RP）转移到其他脂蛋白颗粒的程度。该研究在一个代谢研究单位对3名家族性I型高脂蛋白血症患者、4名脂蛋白脂肪酶（LpL）缺乏的杂合子以及3名对照者进行。给每位受试者给予脂肪负荷，其中I型患者的脂肪负荷占每日总热量的16%，杂合子和对照者为40%，外加60000 U/m²维生素A。对乳糜微粒和非乳糜微粒部分的甘油三酯（TG）和RP水平进行了评估。所有3名I型患者乳糜微粒部分RP清除延迟以及非乳糜微粒RP明显缺乏（推测缺乏残粒产生）表明，RP在胞质内未从乳糜微粒显著转移至现有的载脂蛋白B100颗粒。与正常受试者中TG和RP峰值不一致不同，杂合子的血浆TG和RP曲线是一致的，这与TG和RP具有共同的分解代谢途径一致，而与胞质内RP转移不一致。这证实了关于杂合子中乳糜微粒清除受损的报道。我们得出结论，在LpL缺乏或部分缺乏的个体中，RP是肠道来源颗粒的合适代表性标志物。
