Skarsgard L D, Harrison I
Medical Biophysics Unit, B.C. Cancer Research Centre, Vancouver, Canada.
Radiat Res. 1991 Sep;127(3):243-7.
The dose dependence of the oxygen enhancement ratio (OER) has been examined through multiple measurements of the response of Chinese hamster V79-171 cells to low and high doses of radiation under aerobic and hypoxic conditions. In this series of experiments the cells were maintained at 37 degrees C throughout the gassing and irradiation periods, to simulate normal physiological conditions. Flow cytometry and cell sorting techniques were used to facilitate accurate measurement of cell survival throughout the dose range, but particularly at low dose. The OER was found to decrease significantly at low dose, qualitatively confirming earlier reports from this laboratory, though the decrease was somewhat smaller in the present series. This difference may be a temperature effect since in the earlier experiments irradiation was at 0 degree C. This report shows that the OER decreases from a value of 2.87 +/- 0.16 (standard deviation of mean) at S = 0.01 to 2.36 +/- 0.19 at S = 0.80. Both alpha and beta are altered by the presence of oxygen. The OER is presented as a function of dose in nitrogen.
通过在有氧和缺氧条件下对中国仓鼠V79 - 171细胞对低剂量和高剂量辐射的反应进行多次测量，研究了氧增强比（OER）的剂量依赖性。在这一系列实验中，在通气和辐照期间，细胞始终保持在37摄氏度，以模拟正常生理条件。使用流式细胞术和细胞分选技术来促进在整个剂量范围内准确测量细胞存活率，特别是在低剂量时。发现OER在低剂量时显著降低，定性地证实了本实验室早期的报告，尽管在本系列中降低幅度较小。这种差异可能是温度效应，因为在早期实验中辐照温度为0摄氏度。本报告表明，OER从S = 0.01时的2.87 +/- 0.16（平均值的标准偏差）降至S = 0.80时的2.36 +/- 0.19。α和β都会因氧气的存在而改变。OER表示为氮气中剂量的函数。
