Skarsgard L D, Harrison I, Durand R E
Medical Biophysics Unit, B.C. Cancer Research Centre, Vancouver, Canada.
Radiat Res. 1991 Sep;127(3):248-56.
Flow cytometry and cell sorting techniques have been used together with repeated measurement in an attempt to define better the radiation survival response of asynchronously dividing Chinese hamster V79-171 cells under aerobic and hypoxic conditions. Although the first two decades of cell inactivation have been examined, particular attention has been given to the low-dose range of a few grays, as used in individual radiation therapy treatments. A single linear-quadratic dose-response function was consistently unable to fit both the low-dose and high-dose data satisfactorily, suggesting a two-component response. Separate fitting of the low-dose and high-dose portions of the response yielded alpha and beta values which differed significantly (P = 0.001 to 0.002). The data are consistent with the hypothesis that the observed substructure simply reflects the presence of subpopulations of sensitive (G1-, G2-phase) and resistant (late S-phase) cells, which are resolved in these measurements. These results may have significance for certain situations in radiation therapy and in biophysical modeling of the radiation response.
流式细胞术和细胞分选技术已与重复测量一起使用，试图更好地定义在有氧和缺氧条件下异步分裂的中国仓鼠V79-171细胞的辐射存活反应。尽管已经研究了细胞失活的前二十年，但特别关注了个体放射治疗中使用的几格雷的低剂量范围。单一的线性二次剂量反应函数始终无法令人满意地拟合低剂量和高剂量数据，这表明存在双组分反应。对反应的低剂量和高剂量部分进行单独拟合，得到的α和β值有显著差异（P = 0.001至0.002）。这些数据与以下假设一致，即观察到的亚结构仅仅反映了敏感（G1期、G2期）和抗性（S期后期）细胞亚群的存在，这些亚群在这些测量中得以区分。这些结果可能对放射治疗中的某些情况以及辐射反应的生物物理建模具有重要意义。
