Auersperg N, Hudson J B, Goddard E G, Klement V
Int J Cancer. 1977 Jan;19(1):81-9. doi: 10.1002/ijc.2910190112.
Two-week-old primary cultures of normal adult rat adrenal cortex were exposed to Kirsten murine sarcoma virus (KiMSV). Within a week, the adrenal cells, which are normally fusiform and aligned in parallel, became pleomorphic and piled up extensively. Saturation density increased from 5-10 x 10(4) to 5-10 x 10(5) cells/cm2, population doubling time during exponential growth decreased from 36-40 to 16h, acid production increased and the growth rate became independent of a reduction in serum concentration from 10% to 1%. Inoculation of 2 x 10(6) of these transformed cells into immuno-depressed rats produced rapidly growing tumors within 1 week. Histologically, the tumors were pleomorphic carcinomas with areas ranging from anaplasia to near-normal, highly differentiated adrenocortical tissue. In addition to histologic evidence of differentiation, metabolic studies using 14C-prognenolone showed that the transformed cells were capable of 20alpha reduction and delta5,3beta dehydrogenation, both characteristic of normal steroid-secreting tissues. The transformed adrenocortical cells produced infectious C-type virus as indicated by electron microscopy, 3H-uridine incorporation, and focus formation in NRK (normal rat kidney) cultures. The neutralization pattern of this virus resembled that of authentic Ki-MSV. The transformation of adrenocortical cells by K-MSV demonstrates the capacity of this agent to induce carcinomas in differentiated cells after short-term culture, and widens the range of tissues known to be susceptible to K-MSV to include a secretory epithelium of mesodermal origin.
将正常成年大鼠肾上腺皮质的原代培养细胞培养两周后，使其接触 Kirsten 小鼠肉瘤病毒（KiMSV）。在一周内，原本呈梭形且平行排列的肾上腺细胞变得多形性，并大量堆积。饱和密度从 5 - 10×10⁴个细胞/cm²增加到 5 - 10×10⁵个细胞/cm²，指数生长期的群体倍增时间从 36 - 40 小时减少到 16 小时，酸产量增加，生长速率变得独立于血清浓度从 10%降至 1%的情况。将 2×10⁶个这些转化细胞接种到免疫抑制的大鼠体内，1 周内就产生了快速生长的肿瘤。组织学上，肿瘤为多形性癌，其区域从间变到接近正常、高度分化的肾上腺皮质组织。除了分化的组织学证据外，使用¹⁴C - 孕烯醇酮的代谢研究表明，转化细胞能够进行 20α还原和Δ⁵,3β脱氢，这两者都是正常类固醇分泌组织的特征。如电子显微镜、³H - 尿苷掺入以及在 NRK（正常大鼠肾）培养物中的病灶形成所示，转化的肾上腺皮质细胞产生了传染性 C 型病毒。这种病毒的中和模式类似于正宗的 Ki - MSV。K - MSV 对肾上腺皮质细胞的转化证明了该试剂在短期培养后诱导分化细胞发生癌变的能力，并扩大了已知对 K - MSV 敏感的组织范围，使其包括中胚层来源的分泌上皮。
