Fusco A, Pinto A, Ambesi-Impiombato F S, Vecchio G, Tsuchida N
Int J Cancer. 1981 Nov 15;28(5):655-62. doi: 10.1002/ijc.2910280519.
Fischer rat thyroid epithelial cella (FRT) growing continuously in culture were infected with the Kirsten murine sarcoma virus KiMSV(KiMuLV) and found to produce this virus constitutively. Although the morphology of the FRT cells did not change appreciably, the cells became malignant after infection with KiMSV(KiMuLV) as shown by the growth of infected cels in semi-solid media (uninfected FRT cells did not grow) and by the tumorigenicity of infected when injected into syngeneic animals (uninfected FRT cells or FRT cells infected with non-transforming retro-viruses were not tumorigenic). The induced tumors morphologically resembled moderately differentiated carcinomas. Two markers of thyroid epithelial differentiation were absent in the original FRT clone and remained unexpressed after transformation. Fully differentiated rat thyroid epithelial cells (FRT-L cells) infected with another strain of the Kirsten murine sarcoma virus, the KiMSV(MolMuLV), were also transformed as demonstrated by the ability also of these cells to grow as carcinomas (after in vitro transformation) in syngeneic animals. Our results clearly demonstrate that the Kirsten murine sarcoma virus can transform in vitro cells of epithelial as well as of fibroblastic origin.
在培养中持续生长的费希尔大鼠甲状腺上皮细胞（FRT）被 Kirsten 小鼠肉瘤病毒 KiMSV（KiMuLV）感染，发现其能持续产生这种病毒。尽管 FRT 细胞的形态没有明显变化，但感染 KiMSV（KiMuLV）后细胞变成了恶性细胞，这表现在感染细胞能在半固体培养基中生长（未感染的 FRT 细胞不能生长），以及将感染细胞注射到同基因动物体内具有致瘤性（未感染的 FRT 细胞或感染非转化性逆转录病毒的 FRT 细胞没有致瘤性）。诱导产生的肿瘤在形态上类似于中度分化的癌。甲状腺上皮分化的两个标志物在原始 FRT 克隆中不存在，转化后仍未表达。用 Kirsten 小鼠肉瘤病毒的另一株病毒 KiMSV（MolMuLV）感染完全分化的大鼠甲状腺上皮细胞（FRT-L 细胞），这些细胞也发生了转化，表现为它们在体外转化后也能在同基因动物体内像癌细胞一样生长。我们的结果清楚地表明，Kirsten 小鼠肉瘤病毒能在体外转化上皮来源和成纤维细胞来源的细胞。
