Lydic R, Baghdoyan H A, Lorinc Z
Department of Anesthesia, Pennsylvania State University College of Medicine, Hershey 17033.
Am J Physiol. 1991 Sep;261(3 Pt 2):R766-70. doi: 10.1152/ajpregu.1991.261.3.R766.
Microinjection of cholinergic agonists and acetylcholinesterase inhibitors into the medial pontine reticular formation (mPRF) causes a state that is polygraphically similar to rapid-eye-movement (REM) sleep. Respiratory studies of intact unanesthetized cats during this cholinergically induced REM sleep-like state have shown that the same cholinoceptive pontine reticular regions that mediate REM sleep can also cause state-dependent respiratory depression. The present study investigated the hypothesis that acetylcholine (ACh) release in the mPRF is increased during the respiratory depression that accompanies the cholinergically induced REM sleep-like state. Cats were implanted for polygraphic recording of sleep and wakefulness and with guide tubes aimed for placing a microinjector in one mPRF and a microdialysis probe in the contralateral mPRF. ACh release was measured with high-performance liquid chromatography and electrochemical detection. Compared with waking levels, ACh was significantly increased and respiratory frequency was significantly decreased during the carbachol-induced REM sleep-like state. These results support the hypothesis that endogenous cholinergic neurotransmission in brain regions known to regulate REM sleep can also cause state-dependent changes in respiratory control.
将胆碱能激动剂和乙酰胆碱酯酶抑制剂微量注射到脑桥内侧网状结构（mPRF）中会引发一种状态，该状态在多导睡眠图上与快速眼动（REM）睡眠相似。对完整未麻醉猫在这种由胆碱能诱导的类似REM睡眠状态下进行的呼吸研究表明，介导REM睡眠的相同胆碱能感受性脑桥网状区域也可导致与状态相关的呼吸抑制。本研究调查了以下假设：在伴随胆碱能诱导的类似REM睡眠状态的呼吸抑制过程中，mPRF中的乙酰胆碱（ACh）释放会增加。给猫植入用于多导睡眠图记录睡眠和觉醒的装置，并植入导向管，以便将微量注射器置于一侧mPRF中，将微透析探针置于对侧mPRF中。采用高效液相色谱和电化学检测法测量ACh释放。与清醒水平相比，在卡巴胆碱诱导的类似REM睡眠状态下，ACh显著增加，呼吸频率显著降低。这些结果支持了以下假设：已知调节REM睡眠的脑区中的内源性胆碱能神经传递也可引起呼吸控制中与状态相关的变化。
