Westley A M, Westley J
Department of Biochemistry, University of Chicago, Illinois 60637.
Anal Biochem. 1991 May 15;195(1):63-7. doi: 10.1016/0003-2697(91)90295-5.
A voltammetric method for determining cyanide-reactive sulfane sulfur in biological materials is described. Samples are incubated with a sulfurtransferase, a thiolic cofactor, and cyanide. Thiocyanate formed and/or residual cyanide may then be determined electrochemically with either a silver rotating disk electrode or a dropping mercury electrode in differential pulse mode to provide estimates of sulfane sulfur content. The thiocyanate-based procedure is preferable, particularly when samples contain either serum albumin or inorganic sulfide.
描述了一种用于测定生物材料中氰化物反应性硫烷硫的伏安法。将样品与硫转移酶、硫醇辅助因子和氰化物一起孵育。然后可以使用银旋转圆盘电极或滴汞电极以微分脉冲模式对形成的硫氰酸盐和/或残留的氰化物进行电化学测定，以估算硫烷硫含量。基于硫氰酸盐的方法更可取，特别是当样品含有血清白蛋白或无机硫化物时。
