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[血凝抑制试验:方法的改进、局限性及优势。与中和试验的比较]

[Haemagglutination inhibition test: improvements, limits and advantages of the method. Comparison with neutralization test].

作者信息

Chappuis G, Tixier G

出版信息

Comp Immunol Microbiol Infect Dis. 1982;5(1-3):159-64. doi: 10.1016/0147-9571(82)90030-3.

Abstract

In this study, we report some technical improvements which enable us to envisage wider use of the haemagglutination test and haemagglutination inhibition of the rabies virus. Moderated trypsination of sensitive red blood cells and their counting are important parameters. An interesting application of the test was the haemagglutination competition enabling the amount of soluble glycoprotein (obtained during the fractionating of the virus components) to be evaluated. From antibody kinetics of vaccinated dogs, a study of the correlation which exists between the IHA titres, SN mice titres and SN-RFFIT titres was carried out. Satisfactory correlation was obtained between these three techniques.

摘要

在本研究中,我们报告了一些技术改进,这些改进使我们能够设想更广泛地使用狂犬病病毒血凝试验和血凝抑制试验。对敏感红细胞进行适度胰蛋白酶处理及其计数是重要参数。该试验的一个有趣应用是血凝竞争试验,它能够评估(在病毒成分分级分离过程中获得的)可溶性糖蛋白的量。根据接种疫苗犬的抗体动力学,对间接血凝试验(IHA)效价、小鼠中和试验(SN)效价和快速荧光灶抑制试验(SN-RFFIT)效价之间存在的相关性进行了研究。这三种技术之间获得了令人满意的相关性。

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