改良的Visuwell A群链球菌酶免疫测定法用于检测咽喉拭子中A群链球菌的评估。
Evaluation of the modified Visuwell Strep-A enzyme immunoassay for detection of group-A Streptococcus from throat swabs.
作者信息
Drulak M, Bartholomew W, LaScolea L, Amsterdam D, Gunnersen N, Yong J, Fijalkowski C, Winston S
机构信息
Department of Clinical Microbiology, Buffalo Children's Hospital, New York.
出版信息
Diagn Microbiol Infect Dis. 1991 Jul-Aug;14(4):281-5. doi: 10.1016/0732-8893(91)90017-a.
The modified Visuwell Strep-A enzyme immunoassay (EIA) was compared with culture for detection of group-A Streptococcus from throat swabs. Throat swabs in modified Stuarts medium obtained after culture at two institutions were tested in Visuwell. Cumulative results were n = 417, sensitivity 87.8%, specificity 89.9% predictive value positive (PVP) 67.9%, predictive value negative (PVN) 96.8%, and accuracy 89.5%. At another site, swabs were delivered to the laboratory without transport medium, cultured, and subsequently tested by Visuwell (n = 202, sensitivity 79.6%, specificity 84.5%, PVP 65.2%, PVN 91.9%, accuracy 83.2%). When 1+ culture-positive specimens were considered negative, the sensitivity and PVN increased from 79.6% to 90.2% and 91.9% to 97.1% respectively. Overall performance of the modified Visuwell was comparable with that of the initial assay for throat swabs transported with or without modified Stuarts medium. Cross reaction with organisms other than group-A Streptococcus normally found in the oropharynx was negligible in Visuwell and the limit of detection of group-A Streptococcus was 5 x 10(4) colony-forming units.
将改良的Visuwell A组链球菌酶免疫测定法（EIA）与培养法进行比较，以检测咽喉拭子中的A组链球菌。在两家机构培养后，将取自改良斯图尔特培养基中的咽喉拭子用Visuwell进行检测。累积结果为n = 417，灵敏度87.8%，特异性89.9%，阳性预测值（PVP）67.9%，阴性预测值（PVN）96.8%，准确率89.5%。在另一个地点，拭子未使用转运培养基就被送至实验室，进行培养，随后用Visuwell检测（n = 202，灵敏度79.6%，特异性84.5%，PVP 65.2%，PVN 91.9%，准确率83.2%）。当将1+培养阳性标本视为阴性时，灵敏度和PVN分别从79.6%增至90.2%以及从91.9%增至97.1%。改良的Visuwell的总体性能与初始测定法相当，无论咽喉拭子是否使用改良斯图尔特培养基转运。在Visuwell中，与口咽部通常发现的A组链球菌以外的微生物的交叉反应可忽略不计，A组链球菌的检测限为5×10⁴菌落形成单位。
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