Schwabe L D, Small M T, Randall E L
J Clin Microbiol. 1987 Feb;25(2):309-11. doi: 10.1128/jcm.25.2.309-311.1987.
Results obtained with Abbott Laboratories TestPack Strep A (TPSA), a 7-min enzyme immunoassay method, were compared with culture results to measure the ability of this assay to detect group A streptococci directly from 365 throat swabs. Our study demonstrated a sensitivity of 90.0% and a specificity of 97.4% for TPSA compared with cultures incubated for 48 h. The positive and negative predictive values of this assay versus the culture method were 92.8 and 96.3%, respectively. If specimens that provided fewer than 10 colonies per plate of group A streptococci are eliminated from the data, the sensitivity is increased to 95.6%. Additionally, 10 group A and 40 non-group A streptococcal isolates were tested directly with TPSA for the ability to distinguish group A from non-group A streptococci. All 50 isolates were correctly identified (100% accuracy). TPSA is a rapid, accurate, and easy-to-interpret method for detection and confirmation of group A streptococcal antigen directly from throat swabs and pure culture isolates.
将雅培实验室A群链球菌检测试剂盒（TPSA）（一种需时7分钟的酶免疫测定方法）的检测结果与培养结果进行比较，以评估该检测方法直接从365份咽拭子中检测A群链球菌的能力。我们的研究表明，与培养48小时的结果相比，TPSA的灵敏度为90.0%，特异性为97.4%。该检测方法相对于培养方法的阳性预测值和阴性预测值分别为92.8%和96.3%。如果从数据中剔除每平板A群链球菌菌落数少于10个的标本，则灵敏度可提高到95.6%。此外，直接用TPSA检测了10株A群和40株非A群链球菌分离株区分A群与非A群链球菌的能力。所有50株分离株均被正确鉴定（准确率100%）。TPSA是一种快速、准确且易于解读的方法，可直接从咽拭子和纯培养分离物中检测和确认A群链球菌抗原。
