DeYoung D J, Bantle J A, Fort D J
Department of Zoology, Oklahoma State University, Stillwater 74078.
Drug Chem Toxicol. 1991;14(1-2):127-41. doi: 10.3109/01480549109017872.
The developmental toxicity of five compounds was evaluated with the Frog Embryo Teratogenesis Assay: Xenopus (FETAX) and the results were compared to mammalian literature. Small cell Xenopus laevis blastulae were exposed to ascorbic acid, sodium selenate, coumarin, serotonin and 13-cis retinoic acid for 96 hr. Three separate static-renewal assays were conducted for each compound. Teratogenic potential of the test materials was determined based on Teratogenic Index values [TI = LC50/EC50 (malformation)], types and severity of induced malformations and embryo growth. Ascorbic acid had little or no teratogenic potential. Sodium selenate and coumarin tested as having moderately positive teratogenic potential. Serotonin scored as having moderately strong teratogenic potential and 13-cis retinoic acid scored as having strong teratogenic potential. Results were consistent with mammalian data and support the use of FETAX for the screening of developmental toxicants.
利用非洲爪蟾胚胎致畸试验（FETAX）评估了五种化合物的发育毒性，并将结果与哺乳动物文献进行了比较。将非洲爪蟾小细胞囊胚暴露于抗坏血酸、硒酸钠、香豆素、血清素和13-顺式视黄酸中96小时。对每种化合物进行了三项独立的静态更新试验。根据致畸指数值[TI = LC50/EC50（畸形）]、诱导畸形的类型和严重程度以及胚胎生长情况来确定受试物质的致畸潜力。抗坏血酸几乎没有致畸潜力。硒酸钠和香豆素经测试具有中度正致畸潜力。血清素的致畸潜力评分为中度强，13-顺式视黄酸的致畸潜力评分为强。结果与哺乳动物数据一致，并支持使用FETAX筛选发育毒物。
