Bantle J A, Fort D J, Rayburn J R, DeYoung D J, Bush S J
Department of Zoology, Oklahoma State University, Stillwater 74078.
Drug Chem Toxicol. 1990;13(4):267-82. doi: 10.3109/01480549009032286.
The developmental toxicity of five compounds was evaluated with the Frog Embryo Teratogenesis Assay: Xenopus (FETAX). Late Xenopus laevis blastulae were exposed to 5-azacytidine, methotrexate, pseudoephedrine, aspartame, and amaranth for 96 h. Three separate static-renewal assays were conducted for each compound. Based on Teratogenic Index [LC50/EC50 (malformation)] values, types and severity of induced malformations, and embryo growth, 5-azacytidine and methotrexate tested as having strong teratogenic potential. Pseudoephedrine scored as having moderate teratogenic potential, but amaranth and aspartame had little or no teratogenic potential. Results support the use of FETAX for the screening of developmental toxicants.
采用非洲爪蟾胚胎致畸试验（FETAX）评估了五种化合物的发育毒性。将非洲爪蟾晚期囊胚暴露于5-氮杂胞苷、甲氨蝶呤、伪麻黄碱、阿斯巴甜和苋菜红中96小时。每种化合物进行了三项独立的静态更新试验。根据致畸指数[LC50/EC50（畸形）]值、诱导畸形的类型和严重程度以及胚胎生长情况，5-氮杂胞苷和甲氨蝶呤被检测出具有很强的致畸潜力。伪麻黄碱的致畸潜力为中等，但苋菜红和阿斯巴甜几乎没有或没有致畸潜力。结果支持使用FETAX进行发育毒物的筛选。
