编码山羊生长激素的cDNA的克隆与测序

Cloning and sequencing of cDNA that encodes goat growth hormone.

作者信息

Yamano Y, Oyabayashi K, Okuno M, Yato M, Kioka N, Manabe E, Hashi H, Sakai H, Komano T, Utsumi K

机构信息

Department of Agricultural Chemistry, Kyoto University, Japan.

出版信息

FEBS Lett. 1988 Feb 15;228(2):301-4. doi: 10.1016/0014-5793(88)80020-6.

DOI:10.1016/0014-5793(88)80020-6

PMID:3342884

Abstract

The cDNA that encodes goat growth hormone (gGH) was isolated from a goat pituitary cDNA library. The cDNA, about 880 base pairs long, had a coding sequence, 5'- and 3'-untranslated regions and a poly(A) chain. The cDNA could encode a polypeptide of 217 amino acids. The amino acid sequence homology between gGH and the sequences of bovine GH, rat GH and human GH was 99, 83 and 66%, respectively. By Northern blot hybridization, we found that the possible gGH gene is transcribed in the goat pituitary.

摘要

编码山羊生长激素（gGH）的cDNA是从山羊垂体cDNA文库中分离出来的。该cDNA长约880个碱基对，具有编码序列、5'和3'非翻译区以及一个聚腺苷酸（polyA）链。该cDNA可编码一个由217个氨基酸组成的多肽。gGH与牛生长激素（bGH）、大鼠生长激素（rGH）和人生长激素（hGH）序列之间的氨基酸序列同源性分别为99%、83%和66%。通过Northern印迹杂交，我们发现可能的gGH基因在山羊垂体中被转录。