塞内加尔鳎生长激素编码cDNA的克隆

Cloning of the sole (Solea senegalensis) growth hormone-encoding cDNA.

作者信息

Pendón C, Martínez-Barberá J P, Pérez-Sánchez J, Rodríguez R B, Grenett H, Valdivia M M

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Cádiz, Spain.

出版信息

Gene. 1994 Aug 5;145(2):237-40. doi: 10.1016/0378-1119(94)90012-4.

DOI:10.1016/0378-1119(94)90012-4

PMID:8056337

Abstract

We report here the complete nucleotide (nt) sequence of a cDNA clone encoding Solea senegalensis growth hormone (sGH) isolated from an expression library prepared from sole pituitary gland poly(A)+RNA. The library was screened using a flounder GH cDNA. The cDNA sequence containing an insert of 769 nt was found to encode a polypeptide of 203 amino acids (aa), including a signal peptide of 17 aa. The 5'- and 3'-untranslated regions of the message are 17 and 119-nt long, respectively. Northern blot hybridization detected a 0.9-kb RNA species. The sGH cDNA sequence shows homologies of 80.9, 76.9, 73.8 and 64.2% with the GH of tuna, gilthead seabream, flounder and rainbow trout.

摘要

我们在此报告从塞内加尔鳎垂体腺多聚腺苷酸加尾RNA制备的表达文库中分离出的编码塞内加尔鳎生长激素（sGH）的cDNA克隆的完整核苷酸（nt）序列。该文库使用比目鱼生长激素cDNA进行筛选。发现包含769 nt插入片段的cDNA序列编码一个由203个氨基酸（aa）组成的多肽，其中包括一个17 aa的信号肽。该信使RNA的5'和3'非翻译区分别长17 nt和119 nt。Northern印迹杂交检测到一个0.9 kb的RNA种类。sGH cDNA序列与金枪鱼、金头鲷、比目鱼和虹鳟的生长激素的同源性分别为80.9%、76.9%、73.8%和64.2%。