Drabent B, Kardalinou E, Doenecke D
Institut für Biochemie, Abteilung Molekularbiologie, Georg-August-Universität, Göttingen, Germany.
Gene. 1991 Jul 22;103(2):263-8. doi: 10.1016/0378-1119(91)90284-i.
The gene coding for the human H1t histone, a testis-specific H1 subtype, was isolated from a genomic library using a human somatic H1 gene as a hybridization probe. The corresponding mRNA is not polyadenylated and encodes a 206-amino-acid protein. Sequence analysis and S1 nuclease mapping of the human H1t gene reveals that the 5' flanking region contains several consensus promoter elements, as described for somatic, i.e., S-phase-dependent H1 subtype genes. The 3' region includes the stem-and-loop structure necessary for mRNA processing of most histone mRNAs. Northern blot analysis with RNAs from different human tissues and cell lines revealed that only testicular RNA hybridized with this gene probe.
编码人H1t组蛋白（一种睾丸特异性H1亚型）的基因，是使用人体细胞H1基因作为杂交探针从基因组文库中分离出来的。相应的mRNA没有聚腺苷酸化，编码一种206个氨基酸的蛋白质。人H1t基因的序列分析和S1核酸酶图谱显示，5'侧翼区域包含几个共有启动子元件，如体细胞（即S期依赖性H1亚型基因）中所述。3'区域包括大多数组蛋白mRNA加工所需的茎环结构。用来自不同人体组织和细胞系的RNA进行Northern印迹分析表明，只有睾丸RNA能与该基因探针杂交。
