Anderson D, Soo S S, Towler H
Public Health Laboratory, West Norwich Hospital, Norfolk.
J Clin Pathol. 1991 Aug;44(8):699. doi: 10.1136/jcp.44.8.699.
Acanthamoeba keratitis is an unusual complication of wearing soft contact lenses: a case was diagnosed by culturing corneal scrapings. Acanthamoeba are tolerant of variations in osmolarity in the culture media. Page's saline, recommended for culture agar plates, can easily be substituted with salines which are suitable for cell culture media, thereby avoiding the cumbersome manufacture of special saline. Early diagnosis as a result of successful isolation may avoid the need for keratoplasty.
通过培养角膜刮片诊断出了一例病例。棘阿米巴对培养基中渗透压的变化具有耐受性。推荐用于培养琼脂平板的佩奇氏生理盐水可以很容易地被适合细胞培养基的生理盐水替代,从而避免了特殊生理盐水繁琐的制备过程。成功分离导致的早期诊断可能避免角膜移植的需要。