Yasui Y, Saper C B, Cechetto D F
Department of Pharmacological, University of Chicago, Illinois 60637.
J Comp Neurol. 1991 Jun 8;308(2):293-310. doi: 10.1002/cne.903080212.
The organization of calcitonin gene-related peptide-like immunoreactive (CGRPir) innervation of the amygdala and caudate-putamen in the rat was examined by using immunohistochemistry for CGRP combined with retrograde transport of the fluorescent dye fluoro-gold, as well as anterograde transport of Phaseoleus vulgaris leucoagglutinin (PHA-L). The lateral part of the central nucleus of the amygdala and the amygdalostriatal transition zone was densely innervated by CGRPir terminals at all anterior-posterior levels. More caudally, the lateral part of the caudate-putamen also had large numbers of CGRPir terminals. Injections of fluoro-gold into the amygdala and amygdalostriatal transition area followed by immunohistochemistry for CGRP revealed double-labeled neurons in the subparafascicular, lateral subparafascicular, and posterior intralaminar nuclei of the thalamus and peripeduncular nucleus. Injections into the caudate-putamen demonstrated double-labeled neurons in the more lateral parts of this same nuclear complex. PHA-L injections into the posterior thalamic nuclei from which the CGRPir projections arise confirmed the medial-to-lateral organization of the projections to the amygdala and striatum. The subparafascicular nucleus and the rostral portion of the lateral subparafascicular nucleus primarily projected to the medial amygdala and the amygdalostriatal transition area, while the more lateral cell groups, including the caudal part of the lateral parafascicular, posterior intralaminar, and peripeduncular nuclei projected to the lateral amygdala and the caudate-putamen. These CGRPir projections may be involved in mediating conditioned autonomic and behavioral responses to acoustic stimuli or somatosensory stimuli.
通过使用降钙素基因相关肽样免疫反应（CGRPir）免疫组织化学结合荧光染料氟金的逆行运输以及菜豆白细胞凝集素（PHA-L）的顺行运输，研究了大鼠杏仁核和尾状核 - 壳核中CGRPir神经支配的组织情况。在所有前后水平上，杏仁核中央核的外侧部分和杏仁核纹状体过渡区均被CGRPir终末密集支配。更靠尾侧的部位，尾状核 - 壳核的外侧部分也有大量CGRPir终末。向杏仁核和杏仁核纹状体过渡区注射氟金后进行CGRP免疫组织化学检测，发现在丘脑的束旁下核、外侧束旁下核和后内侧核以及脚周核中有双标记神经元。向尾状核 - 壳核注射显示在同一核复合体的更外侧部分有双标记神经元。向发出CGRPir投射的丘脑后核注射PHA-L证实了向杏仁核和纹状体投射的内侧到外侧的组织方式。束旁下核和外侧束旁下核的 Rostral部分主要投射到内侧杏仁核和杏仁核纹状体过渡区，而更外侧的细胞群，包括外侧束旁核的尾侧部分、后内侧核和脚周核投射到外侧杏仁核和尾状核 - 壳核。这些CGRPir投射可能参与介导对听觉刺激或体感刺激的条件性自主神经和行为反应。
