Ohno H
Section of Hematology, Tenri Hospital.
Rinsho Ketsueki. 1991 Jun;32(6):655-9.
The 14;19 translocation [t(14;19) (q32;q13)] is a recurring chromosomal translocation observed in leukemic cells of chronic lymphocytic leukemia showing prolymphocytic transformation. We have cloned the breakpoint junction of the translocation and identified a new gene, bcl-3, on the chromosome band 19q13 adjacent to the breakpoint. The translocation occurred at the switch region of the alpha constant locus of the immunoglobulin heavy chain gene and the upper stream of the bcl-3 gene, resulting in a head-to-head recombination between the two genes on the 14q+ chromosome. The bcl-3 gene was highly expressed in the leukemic cells carrying the 14;19 translocation. The bcl-3 gene product contained repeat structures found in proteins involved in cell cycle control and cell lineage determination. These results suggest that the bcl-3 is a proto-oncogene that may contribute to the development of leukemias carrying the 14;19 translocation.
14;19易位[t(14;19)(q32;q13)]是在慢性淋巴细胞白血病出现幼淋巴细胞转化的白血病细胞中观察到的一种反复出现的染色体易位。我们克隆了该易位的断点连接区，并在靠近断点的19q13染色体带上鉴定出一个新基因bcl-3。易位发生在免疫球蛋白重链基因α恒定区的转换区与bcl-3基因的上游之间，导致14q+染色体上这两个基因之间发生头对头重组。bcl-3基因在携带14;19易位的白血病细胞中高度表达。bcl-3基因产物含有在参与细胞周期调控和细胞谱系确定的蛋白质中发现的重复结构。这些结果表明，bcl-3是一种原癌基因，可能在携带14;19易位的白血病发生中起作用。
