McKeithan T W, Takimoto G S, Ohno H, Bjorling V S, Morgan R, Hecht B K, Dubé I, Sandberg A A, Rowley J D
Department of Pathology, University of Chicago, IL 60637, USA.
Genes Chromosomes Cancer. 1997 Sep;20(1):64-72.
The t(14;19)(q32.3;q13.1) is a recurring translocation found in the neoplastic cells of some patients with chronic lymphocytic leukemia (CLL) or other B-lymphocytic neoplasms. We previously cloned the translocation breakpoint junctions present in the leukemic cells from three such patients and identified a gene, BCL3, whose transcription is increased as a result of the translocation. In the present paper, we describe three additional patients with the t(14;19), one with lymphoma and two with CLL, and report the cloning and sequencing of the breakpoint junction in one of these patients as well as in a previously reported patient. We and others have found that the breakpoints on chromosome 14, with one exception, fall within the switch region upstream of the immunoglobulin heavy chain C alpha 1 or C alpha 2 sequences. Several of the breaks within chromosome 19 fall immediately upstream of the BCL3 gene, but several others are more than 16 kb 5' of the gene. Most patients with CLL and the t(14;19) also show trisomy 12.
t(14;19)(q32.3;q13.1)是在一些慢性淋巴细胞白血病（CLL）患者或其他B淋巴细胞肿瘤患者的肿瘤细胞中发现的一种反复出现的易位。我们之前克隆了来自三名此类患者白血病细胞中的易位断点连接，并鉴定出一个基因BCL3，其转录因易位而增加。在本文中，我们描述了另外三名患有t(14;19)的患者，一名患有淋巴瘤，两名患有CLL，并报告了其中一名患者以及一名先前报道患者的断点连接的克隆和测序情况。我们和其他人发现，14号染色体上的断点，除了一个例外，都落在免疫球蛋白重链Cα1或Cα2序列上游的转换区域内。19号染色体上的几个断点紧邻BCL3基因上游，但其他几个断点在该基因5'端上游超过16 kb处。大多数患有CLL和t(14;19)的患者也显示12号染色体三体。
