Yabumoto K, Ohno H, Doi S, Edamura S, Arita Y, Akasaka T, Matsumoto J, Kadowaki N, Fukuhara S, Okuma M
First Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Int J Hematol. 1994 Apr;59(3):211-8.
The t(14;19)(q32;q13) is a recurring translocation found in some patients with chronic lymphocytic leukemia (CLL), and the t(14;19) juxtaposes the BCL3 gene on chromosome 19 with the immunoglobulin heavy chain gene (IGH) locus on chromosome 14. Genomic DNAs from 49 patients with chronic B-cell leukemia and the related lymphomas were examined by Southern blot hybridization using 2 separate probes, named p alpha 1.4P and p alpha .5B, from the BCL3 gene locus. None of the 18 patients with leukemic manifestations of non-Hodgkin's lymphomas had detectable BCL3 rearrangements. Of 31 patients with CLL, 2 had the BCL3 rearrangements. A comigration study using the C alpha and C epsilon constant gene probe from IGH indicated that the t(14;19) translocation occurred in these 2 patients, and they were diagnosed with CLL/prolymphocytic (PL) according to the French-American-British (FAB) classification. Probes for the IGH locus revealed that leukemia cells of the 2 patients each were clonal, indicating that both small lymphocytes and prolymphocytoid cells found in the peripheral blood of one patient had the t(14;19), as well as a major population of the small lymphocytes in the peripheral blood of a second patient. It thus appears that tumor cells carrying the t(14;19) constitute a distinct disease entity in a group of chronic B-cell leukemia, that has a converting potential to more aggressive forms.
t(14;19)(q32;q13)是在一些慢性淋巴细胞白血病（CLL）患者中发现的一种反复出现的易位，t(14;19)使19号染色体上的BCL3基因与14号染色体上的免疫球蛋白重链基因（IGH）位点并列。使用来自BCL3基因位点的2个单独探针p alpha 1.4P和p alpha.5B，通过Southern印迹杂交对49例慢性B细胞白血病及相关淋巴瘤患者的基因组DNA进行检测。18例非霍奇金淋巴瘤白血病表现患者中均未检测到BCL3重排。在31例CLL患者中，2例有BCL3重排。使用来自IGH的C alpha和C epsilon恒定基因探针进行的共迁移研究表明，这2例患者发生了t(14;19)易位，根据法国-美国-英国（FAB）分类，他们被诊断为CLL/幼淋巴细胞白血病（PL）。IGH位点的探针显示，这2例患者的白血病细胞均为克隆性，表明1例患者外周血中发现的小淋巴细胞和幼淋巴细胞样细胞均有t(14;19)，另一例患者外周血中的主要小淋巴细胞群也有t(14;19)。因此，携带t(14;19)的肿瘤细胞似乎在一组慢性B细胞白血病中构成了一个独特的疾病实体，具有向更具侵袭性形式转化的潜力。
