5' termini of poliovirus RNA: difference between virion and nonencapsidated 35S RNA.
作者信息
Fernandez-Muñoz R, Lavi U
出版信息
J Virol. 1977 Feb;21(2):820-4. doi: 10.1128/JVI.21.2.820-824.1977.
Abstract
Poliovirus cytoplasmic, nonencapsidated 35S RNA yields approximately one pUp per molecule upon T2 RNase digestion, indicating that this RNA has the same 5' end as the polyribosome-associated viral RNA fraction. Double-stranded, replicative form RNA after the same treatment yielded approximately four pNp structures per molecule, 65% of which was pUp. In contrast, the 35S RNA from mature virions contained no detectable pNp, indicating that the 5' end of the virion RNA is different from that of the nonencapsidated RNA. None of the above molecules contained pppNp, ppNp, or GpppNp structures present in host mRNA. The virion RNA molecules, as we have shown previously for thenonencapsidated 35S viral RNA (Fernandez-Muñoz and Darnell, 1976), is not labeled with [methyl-3H]methionine.
摘要
相似文献
1
5' termini of poliovirus RNA: difference between virion and nonencapsidated 35S RNA.
J Virol. 1977 Feb;21(2):820-4. doi: 10.1128/JVI.21.2.820-824.1977.
2
The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80. doi: 10.1073/pnas.73.2.375.
3
Polyadenylic acid on poliovirus RNA. II. poly(A) on intracellular RNAs.
J Virol. 1975 Jun;15(6):1418-31. doi: 10.1128/JVI.15.6.1418-1431.1975.
4
Polyadenylic acid on poliovirus RNA. III. In vitro addition of polyadenylic acid to poliovirus RNAs.
J Virol. 1975 Jun;15(6):1432-9. doi: 10.1128/JVI.15.6.1432-1439.1975.
5
Possible point mutation sites in LSc, 2ab poliovirus RNA and a protein covalently linked to the 5'-terminus.
J Gen Virol. 1979 Oct;45(1):107-17. doi: 10.1099/0022-1317-45-1-107.
6
Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.
Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5. doi: 10.1073/pnas.74.3.961.
7
Two forms of VPg on poliovirus RNAs.
J Virol. 1981 Jun;38(3):863-71. doi: 10.1128/JVI.38.3.863-871.1981.
8
Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis.
J Virol. 1976 May;18(2):719-26. doi: 10.1128/JVI.18.2.719-726.1976.
9
Polyadenylic acid at the 3'-terminus of poliovirus RNA.
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1877-82. doi: 10.1073/pnas.69.7.1877.
10
Polyadenylic acid sequences in the virion RNA of poliovirus and Eastern Equine Encephalitis virus.
Science. 1972 May 5;176(4034):526-8. doi: 10.1126/science.176.4034.526.
引用本文的文献
1
Encapsidation of Viral RNA in : Studies on Cowpea Mosaic Virus Demonstrate Dependence on Viral Replication.
J Virol. 2019 Jan 4;93(2). doi: 10.1128/JVI.01520-18. Print 2019 Jan 15.
2
Divergent Requirement for a DNA Repair Enzyme during Enterovirus Infections.
mBio. 2015 Dec 29;7(1):e01931-15. doi: 10.1128/mBio.01931-15.
3
Engineered picornavirus VPg-RNA substrates: analysis of a tyrosyl-RNA phosphodiesterase activity.
PLoS One. 2011 Mar 7;6(3):e16559. doi: 10.1371/journal.pone.0016559.
4
RNA binding properties of poliovirus subviral particles.
J Virol. 1995 Jan;69(1):13-22. doi: 10.1128/JVI.69.1.13-22.1995.
5
Picornaviral structure and assembly.
Microbiol Rev. 1981 Jun;45(2):287-315. doi: 10.1128/mr.45.2.287-315.1981.
6
The 5'-untranslated region of picornaviral genomes.
Adv Virus Res. 1991;40:103-80. doi: 10.1016/s0065-3527(08)60278-x.
7
Capsid protein precursor is one of two initiated products of translation of poliovirus RNA in vitro.
J Virol. 1979 May;30(2):481-8. doi: 10.1128/JVI.30.2.481-488.1979.
8
Identification of specific fragments containing the 5' end of poliovirus RNA after ribonuclease III digestion.
Nucleic Acids Res. 1978 Nov;5(11):4039-54. doi: 10.1093/nar/5.11.4039.
9
The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5345-9. doi: 10.1073/pnas.74.12.5345.
10
Encephalomyocarditis virus RNA. III. Presence of a genome-associated protein.
J Virol. 1978 Jan;25(1):413-5. doi: 10.1128/JVI.25.1.413-415.1978.
本文引用的文献
1
THE EXTRACTION OF RIBONUCLEIC ACID FROM POLIOVIRUS BY TREATMENT WITH SODIUM DODECYL SULFATE.
Virology. 1964 Mar;22:360-7. doi: 10.1016/0042-6822(64)90026-1.
2
The time course of synthesis of poliovirus RNA.
Virology. 1961 Mar;13:271-9. doi: 10.1016/0042-6822(61)90145-3.
3
Purification and properties of the replicative intermediate of the RNA bacteriophage R17.
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504-11. doi: 10.1073/pnas.55.6.1504.
4
A circula DNA-protein complex from adenoviruses.
Virology. 1973 Nov;56(1):54-69. doi: 10.1016/0042-6822(73)90287-0.
5
Morphogenesis of poliovirus. II. Demonstration of a new intermediate, the proviron.
J Virol. 1973 Nov;12(5):1122-30. doi: 10.1128/JVI.12.5.1122-1130.1973.
6
Sequence studies of poliovirus RNA. I. Characterization of the 5'-terminus.
J Mol Biol. 1972 Jul 28;68(3):537-40. doi: 10.1016/0022-2836(72)90106-4.
7
Initiation of polyribosome formation in poliovirus-infected HeLa cells.
J Mol Biol. 1970 Feb 14;47(3):275-91. doi: 10.1016/0022-2836(70)90302-5.
8
Molecular weight of poliovirus ribonucleic acid.
J Virol. 1969 Oct;4(4):475-9. doi: 10.1128/JVI.4.4.475-479.1969.
9
Infectious replicative intermediate of poliovirus: purification and characterization.
Virology. 1969 Apr;37(4):521-34. doi: 10.1016/0042-6822(69)90270-0.
10
Aspects of the synthesis of poliovirus RNA and the formation of virus particles.
Virology. 1966 Jun;29(2):179-89. doi: 10.1016/0042-6822(66)90024-9.
Zotero 插件