脊髓灰质炎病毒RNA的5'末端：病毒粒子与非衣壳化35S RNA之间的差异
5' termini of poliovirus RNA: difference between virion and nonencapsidated 35S RNA.
作者信息
Fernandez-Muñoz R, Lavi U
出版信息
J Virol. 1977 Feb;21(2):820-4. doi: 10.1128/JVI.21.2.820-824.1977.
Abstract
Poliovirus cytoplasmic, nonencapsidated 35S RNA yields approximately one pUp per molecule upon T2 RNase digestion, indicating that this RNA has the same 5' end as the polyribosome-associated viral RNA fraction. Double-stranded, replicative form RNA after the same treatment yielded approximately four pNp structures per molecule, 65% of which was pUp. In contrast, the 35S RNA from mature virions contained no detectable pNp, indicating that the 5' end of the virion RNA is different from that of the nonencapsidated RNA. None of the above molecules contained pppNp, ppNp, or GpppNp structures present in host mRNA. The virion RNA molecules, as we have shown previously for thenonencapsidated 35S viral RNA (Fernandez-Muñoz and Darnell, 1976), is not labeled with [methyl-3H]methionine.
摘要
脊髓灰质炎病毒的细胞质非衣壳化35S RNA经T2核糖核酸酶消化后，每个分子产生约一个pUp，这表明该RNA的5'端与多核糖体相关病毒RNA组分的5'端相同。相同处理后的双链复制型RNA每个分子产生约四个pNp结构，其中65%是pUp。相比之下，成熟病毒粒子的35S RNA未检测到pNp，这表明病毒粒子RNA的5'端与非衣壳化RNA的5'端不同。上述分子均不含有宿主mRNA中存在的pppNp、ppNp或GpppNp结构。正如我们之前对非衣壳化35S病毒RNA所表明的那样（费尔南德斯-穆尼奥斯和达内尔，1976年），病毒粒子RNA分子未被[甲基-3H]甲硫氨酸标记。
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