Banati R B, Rothe G, Valet G, Kreutzberg G W
Department of Neuromorphology, Max-Planck-Institute for Psychiatry and Cellular Biochemistry Group, Martinsried, FRG.
Neuropathol Appl Neurobiol. 1991 Jun;17(3):223-30. doi: 10.1111/j.1365-2990.1991.tb00718.x.
A new flow cytometric method for the investigation of the respiratory burst of macrophages/microglia isolated from neonatal rat brain has been established. Respiratory burst activity was measured quantitatively in single viable cells by the intracellular oxidation of non-fluorescent dihydrorhodamine 123 (DHR) to fluorescent rhodamine 123. Cultured microglia exhibited high spontaneous respiratory burst activity already before stimulation. After maximal stimulation with phorbol myristate acetate, DHR oxidation rose by 40-95%. The respiratory burst activity in resident or inflammatory, i.e. thioglycolate elicited, peritoneal macrophages was significantly lower than in cultured brain macrophages suggesting a high potential of microglia for oxidative tissue destruction.
已建立一种新的流式细胞术方法，用于研究从新生大鼠脑部分离的巨噬细胞/小胶质细胞的呼吸爆发。通过将非荧光二氢罗丹明123（DHR）细胞内氧化为荧光罗丹明123，对单个活细胞中的呼吸爆发活性进行定量测量。培养的小胶质细胞在刺激前就已表现出较高的自发呼吸爆发活性。在用佛波酯肉豆蔻酸酯乙酸盐进行最大刺激后，DHR氧化增加了40%-95%。驻留或炎症性（即由巯基乙酸盐引发的）腹腔巨噬细胞中的呼吸爆发活性明显低于培养的脑巨噬细胞，这表明小胶质细胞具有很高的氧化组织破坏潜力。
