Pruimboom W M, van Dijk A P, Tak C J, Zijlstra F J, Bonta I L, Wilson J H
Department of Pharmacology, Erasmus University, Rotterdam, The Netherlands.
Agents Actions. 1993;38 Spec No:C86-8. doi: 10.1007/BF01991146.
Human peritoneal macrophages (hp-M phi) are a source of inflammatory mediators. After stimulation in vitro for 24 h with LPS there was a significant increase in cytokine production (IL-1, IL-6 and TNF alpha), but not in the production of eicosanoids from endogenous arachidonate. Leukotrienes are the predominant eicosanoids formed after stimulation with calcium ionophore for 15 min, while prostaglandin formation is insignificant. The fluorescence intensity of TPA-stimulated and DHR123 loaded hp-M phi (a measure of the respiratory burst) increases significantly in a short period of time. Hp-M phi will be useful as a model for testing the effects of anti-inflammatory drugs on eicosanoid and cytokine production and respiratory burst activity in vitro.
人腹膜巨噬细胞（hp-M phi）是炎症介质的一个来源。用脂多糖（LPS）在体外刺激24小时后，细胞因子（白细胞介素-1、白细胞介素-6和肿瘤坏死因子α）的产生显著增加，但内源性花生四烯酸生成类花生酸的量未增加。在用钙离子载体刺激15分钟后形成的主要类花生酸是白三烯，而前列腺素的形成不明显。佛波酯（TPA）刺激并用二氢罗丹明123（DHR123）负载的hp-M phi的荧光强度（呼吸爆发的一个指标）在短时间内显著增加。hp-M phi将作为一种模型，用于测试抗炎药物对体外类花生酸和细胞因子产生以及呼吸爆发活性的影响。
